| An issue should be resolved urgently is that the dental implants are vulnerable to inflammation and hard to heal with the bone after implanted in vivo. In this paper,chitosan/polylysine with a better antibiosis effect and alginate which promotes endothelial cell adhesion and growth wereassembled onto titanium surface by a layer-by-layer(LBL) assembly and electrophoresis technique.Both the antibiosis compatibility and the cell compatibility of titanium were anticipated to improvethrough the combination of tiiese two biomolecules.Titanium by micro-arc oxidation(MAO) technology and then activated by Na OH solution,which generated a negatively charged hydroxyl-rich surface. Subsequently, poly-L-lysine(PLL)was bound and thereby formed positively charged amino group modified surface. Then thenegatively charged chitosan and the positively charged alginate were alternately assembled onto the titanium surface to form the functional multilayers.The results of FTIR and the quantitative characterization of heparin showed that the Chitosan and alginate were successfully assembled onto the activated titanium surface. The roughness and the hydrophilic property of the functional multilayer smnple increase after assembled functional multilayer on Ti which were characterized by SEM, AFM and water contact angle test.The results of osteoblast static culture in vitro indicated that the modified surface significantly promoted more endothelial progenitor cells adhesion, better cell growth and proliferation.All tiese results demonstrated that the chitosan and alginate assembly multilayer not only improved the antibiosis compatibility to a certain extent, but also significantly improved the cell compatibility. It was shown that this method using two ftmctional biomolecules might provide a reference for surface modification of dential implant. |
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