Research On Extraction, Purification And Complement Fixating Activity Of Polysaccharides In Kelp Residue

China is a big maritime power. The strategic conception of "21st Century the Silk Road on the Marit ime " sets a goal to promote our country from a big marine power to the powerful marine power. Thus, it is strategically important to develop the marine econo my, improve the level of marine scientific research, and recycle marine biomass. The studies make the waste residue generated from kelp processing industry as raw materials. The feasibility of kelp residue biomass resources utilization was studied and evaluated. We are looking forward to turning ―waste‖-the kelp slag- into treasure. This method can both overcome the problem of environmental pollut ion it causes and bring higher economic benefits.Firstly, we used kelp residue as material to study the optim ization of polysaccharide-extracting technology of seaweed. The uniform design method was used to design the project, and during the algal polysaccharides extraction process, the influence of different extraction temperature, extraction p H, extraction duration, extraction times and different solid to liquid ratio for polysaccharide extraction rate was studied. Based on different optimization model and mult ip le regression analys is methods, the alga l polysaccharides extraction process which took polysaccharide extraction yie ld as the optimizing index c ould be determined: the extraction temperature was 123.7℃, extraction p H was 8.25, and the extraction duration was 96.6 minutes, repeated three times and material- water ratio was 1:9.7. The predicted value of the crude polysaccharide extraction rate was 2.1 9% in this model, while the test data was 2.44 %, and the extraction rate of optimized polysaccharide was improved by 50% comparing with the untreated one, which was significantly higher than the simultaneous setting extraction method of the four groups.Secondly, upon the uniform design method, the complement binding activity of the polysaccharide was measured and the project was optimized according to the ICH50 values of the complement binding activity. The results showed that the crude polysaccharides of the kelp residue all had good complement activity in different uniform design projects. The lowest IC H50 value was 5.6873μg/m L and the maximum was 47.7665μg/m L. The optimization model was established based on the index of complement binding activit y, the predicted and the actual test results of different regression analysis models showed that partial regression analys is had the best effect. And it turned out that binding activity of algal polysaccharides extracted from the optimization model had been further improved.Crude polysaccharide in kelp residue was separated and purified using column chromatography. First, we did the dynamic adsorption screening of four kinds of macroporous resin, and the D3520 macroporous resin was fina lly determined for subsequent experiments. After purification by D3520, the actual recovery rate of polysaccharide was 62.4%, the removal rate of pigment was 65.0%, the protein removal rate was 70.1%, and the purity of polysaccharide increased 2.38 times. After purification by ion exchange resin DEAE Sepharose C L-6B, the crude polysaccharide was divided into 10 different polysaccharide fractions. The total polysaccharide recovery was reached to 69. 94%, and the purity of the main components was close to 80%. We cho se HD-7 and HD-8 for further purification study by Sephadex- G75, and the results showed that the two components were further separeated into two different polysaccharide components.In summary, We established a comprehensive method of balancing kelp residue extraction rate of polysaccharide and complement binding activity, then found a method to separate and purify the crude polysaccharide of kelp residue. A new way and the scientific basis for the utilization of kelp residue can be obtained from this issue. It is powerful to provide a useful basis for the development and application of the bioactive polysaccharide.