The Synthesis And Characterization Of ZnO Nanocrystals And The Study Of Antibacterial Activity

Using of large amounts of excess of penicillin treatment of various bacterial infections, super bacteria cause about human deaths happened from time to time. Researchers are looking for a new type of antimicrobial agent which can well drug-resistant. At present, Zn O nanoparticles as an important part of inorganic nanomaterials have been widely used in the field of antibacterial agents.However, the synthesis of Simple and cheap, water stability, monodispersity, biocompatibility and small size Zn O nanocrystal is still challenging. At the same time, Zn O nanocrystals reported as for antimicrobial agent in vivo are rare. The study of antibacterial of Zn O nanocrystals in vivo will provide theoretical guidance and reference to the research on the antibacterial agent in vivo in the future.In the project, small molecule ligands solvothermal methods are used to prepare the Zn O nanocrystals with well monodispersion hydrophilic. The structure, size, composition, surface properties and optical properties would be evaluated.Second, the biocompatibility of zinc oxide nanocrystals will be evaluated. The mechanism of zinc oxide nanocrystals leading to Hep G-2 cell apoptosis will be researched. Biological safety is evaluated by acute toxicity and hemolytic assay.Third, inhibition zone and minimum inhibitory concentration(MIC) assay are used to evaluate the activity of antibacterial of Zn O nanocrystals. Vaginitis model is applied to study the activity of antibacterial in vivo. Zn O nanocrystals have well the activity of antibacterial and low toxicity in vivo.