Computational Mathematics In The Xylene Isomers Chromatography Overlapping Peaks Resolve Application

Chromatography has been used extensively in respect of monitoring atmosphere, quality of water, soil, living things, food, environmental contaminant and so on, and some chromatographic overlapping peaks were met often, that is difficult to be separated even if the cbromatographic condition be changed. Some time, monitors and studies were restricted greatly. In this research, Kalman filtering and ridge regression mathematical methods were first introduced to determine xylene isomers and a solution for xylene isomers chromatographic overlapping peak under certain experimental condition was studied. For this reason, it become possible that xylene isomers or other compounds whom it is difficult to separate can be determined quantitatively by using a common chromatographic column in a simple chromatographic condition.A little error of experimental data can make a big error of the final result. Therefore, some conditions whose can effect the stability of chromatographic signal were observed and studied to ensure accurate result. The experiment showed that a high stable power transformer is necessary and a single but dual bump is necessary too to reduce the unstable chance~. Every solution should be made up as an experiment is ready and the volatilizes of solutions must be avoided.We used 6A liquid chromatography, N2000 chromatographic working-station and a program of selecting data to collect data. The result was obtained for us to operate the collected data in a Kalman filtering program or a ridge regression program. To obtain a better result, the research was done for selecting data, so that a reference point was selected to avoid the effect of chromatographic peak displacement and a better range and interval of time were looked for. The research showed that it is better for the reference point to be closed to the chromatographic peak of detected components or to be the top point of I ,2-xylene chromatographic peak and for selected data to be in the whole range of the chromatographic peak of xylene isomers.The result showed that the relative error of every sample made up are less 8%and the average rate of recovery of every component reach 91?06 Va. The differentiation of chromatographic overlapping peak is satisfactory.