Analysis Of Soy Lecithin And Refined Methods Of Research,

In this text, we studied the separation, purification and determination technology of high purity soybean phosphatidylcholine.A simple rapid and accurate quantitative method was established for the determination of lecithin by HPLC-RI in this research. Separation was obtained by using Waters Spherisorb S5W (5μm Silica) 4.6 X 250 mm silica column and mobile phase of n-hexane:2-propanol:water(l:4:l,v/v/v) with flow rate of 0.8ml/min. Under this condition, the retention time of PC was about 11 minutes. This method produced data which correlated well with data from the TLC-P method, and this data was highly accurate and exhibited satisfactory reproducibility.The soy lecithin with 40-50% PC was obtained from soybean raw material by using acetone and 95% ethanol, the yield of PC in this process was about 65%.High purity soybean phosphatidylcholine can be obtained in this method: first PE LPC SM, etc was separated from PC by silica column chromatography, then the product of silica column chromatography was treated by alumina column chromatogr-aphy, PI can be removed and product with 94.51% PC can be obtained. The yield of this process was 90.88%.The mobile phase of silica column chromatography: mixed solvent of petroleum ether (60~90℃), ethanol and water(l:l:0.175); the mobile phase of alumina column chromatography :95% ethanol.