The Curcuma Cell Suspension Culture, Volatile Oil, The Separation Of Polysaccharide And Polysaccharide Biological Activity

The high-yield cell suspension line of Curcuma Zedoaria (Berg.) Rosc was established with yellow and loose small callus inoculated into different liquid medium. These conditions for cell growth and volatile oil and polysaccharides synthesis well were selected, such as carbon source, nitrogen source, hormone, pH, rotation speed of table, inoculation concentration, subculture period, light/dark circle and so on. The cells were collected after a certain culture period. The volatile oil content was determined by steam distillation method. The polysaccharide content was determined with PhenyIhydrate-Sulfuric acid. The precursors of Ammonium acetate and Potassium acetate and Calcium Pantothenate and energy substances of ATPNa2 were added into the volatile oil synthesis-controlling condition.The results showed that the cell growth enhanced 1.82 times contrast to the CK group. The polysaccharides content was 45.53% which was 1.37 times of it in herbal slice .The volatile oil content was 2.62% and it's 1.71 times of CK group. These studies were not reported and provided theoretical base for industrializing.The crude polysaccharides was extracted from herbal slice of Curcuma zedoaria Rosc with hot water ,and precipitated by ethanol after concentration. The constituents were isolated by DEAD-52 column chromatography. The water effluent was isolated. Its purification was detected by Sepharose electrophoresis. The bioactivities of immunology, anti-oxidative function and antitumor activity of the polysaccharides in vivo or vitro were studied. The results showed that the content of total polysaccharides was 33.12%. A higher antitumor effect of the polysaccharides by injection was observed, and the effect was dose-dependent. The inhibitive rate was 54.78% when the dose at 500mg.·kg-1·day-1. The polysaccharides could raise the spleen cell proliferation and transformation rate of lymphocyte and the activity of SOD in blood of mice. The polysaccharides could scavenge ?OH . The ?OH scavenging ratio was 36.56% at the density of 100mg/mL. The polysaccharides could raise the immune regulation and the activities of SOD, having the anti-oxidation activity had a higher antitumor effect on H22 tumor .The orthogonal design method was used to establish the optimum condition for extracting polysaccharide and volatile oil at the same time. The result showed that the herbal slices were pulverized in 200 orders, and was extracted 4 hours and with solution 1∶20 could obtain the highest extraction amount, which was 24.8%and 2.21%.