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Commonly Used Chemical Pesticides On Rice Physiological And Biochemical Effects And Mechanism Studies

Posted on:2005-06-01Degree:MasterType:Thesis
Country:ChinaCandidate:X M FengFull Text:PDF
GTID:2191360125952783Subject:Biophysics
Abstract/Summary:
Effects of common pesticides on the physiology and biochemistry in different rice varieties were studied in this paper. The results were showed as follows:1. Study on tracer dynamics of effects of pesticides on photosynthate export rate of in rice leafEffects of three pesticides, triazophos, jingganmycin and bisultap, on export rate of photosynthate of rice leaf were studied with 14C on rice varieties, spraying time and application concentration. Dynamics data of photosynthate of labeled rice functional leaves of different treatments were recorded with living plant nutrient detection instrument equipped with multi-probes through tracer method of radioactive nuclide. Compartment analysis model and mathematical equations were constructed with dynamics principle and method of analysis, calculating constant (K) of export rate. The result showed that export rate of photosynthate of labeled leaf was reduced after treatments with pesticides. This effect was positively correlated with decline of photosynthate export rate of the leaf and mainly attributed to the reduction of photosynthesis rate.2. Effects of Jinggangmycin and Triazophos on Malondialdehyde (MDA) and Chlorophyll contents in rice leaf bladesChanges of MDA and Chlorophyll contents in rice leaf blades after different dosage of two pesticides,Jinggangmycin and Triazophos treatments were studied.The results showed that the content of MDA and chlorophyll in rice leaves were significantly affected by the two pesticides. MDA content and residual time of pesticides was positively correlated with the dose of the pesticides. However , on the contrary , effects of pesticides on chlorophyll content was negatively correlated with the dose.3.Effects of several pesticides on SOD activity and amounts of free amino acids in rice .The Effects of several pesticides on SOD actively in different rice varieties werestudied .The results showed that almost all the herbicide treatments on different rice varieties increased the SOD activity during 15 days after first trestment (DAT).SOD activity of rice plant reached maximum on 10 DAT and began to decline on 15 DAT ,and then recovered to control level on 21 DAT. SOD activity of rice plant after two applications of pesticides (using insecticide-bisultap or fungicide-jinggang mycin on 22 DAY after herbicide treatments ) increased in comparison with that of the control on 2 DAY after second treatment and declined on 6 DAY, indicating that twe applications of pesticides had more serious impact on rise plant compared with once application of pesticides. SOD activity of rice plant may be an index of rice plant against the stress.The results also showed that amounts of the amounts of free amaino acids increased ,but it didn't increase significantly in compared with the control. Change of some amino acid was the main cause of decreasing of plant rice .4.Effects of common pesticides on the amount of oxalic acid, reducing sugar and glutathione- S -transferase .Effects of common pesticides on physiology and biochemistry of three rice varieties were studied .The result showed that all of the 5 pesticides caused decline of the amount of oxalic acid on the third day after treatments. The effect of pesticides on Xiushuill9 and Zhendao2 was more serious than that on Xiushui63. However , there was no obvious effect on reducing sugar content in rice leaf . The effects of pesticides on glutathione-S-transferase (GST) also varied among pesticides and rice varieties .The effect of pesticides on Zhendao2 and XiushuillO were greater than on Xiushui 63, but for Zhendao2 , GSTs after trentment with imidacloprid jinggangmycin ,bisultap increased significantly ,while inversely for triazophos treatment .Buprofezin treatment had little effect on GST of rice plant.5. Effects of pesticides on growth and ultraweak bioluminescence in rice seedling. The effects of such pesticides as above on growth and ultraweak bioluminescencein different rice seedling varieties were studied . The results showed that both 2 pesticides affected growth...
Keywords/Search Tags:Physiological
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