Chromatography Split Several Chiral Drugs

The reason of development of chiral technologies was analyzed, and existing chiral separation methods and their advantages and disadvantages were reviewed detailedly in this paper. The enantiomers of three chiral drugs were separated individually using chromatographic methods. Firstly, the research of resolution of chlorthalidone enantiomers with high performance liquid chromatography using hydropropyl-β-cy-clodextrin as mobile phase additive was further studied, the factors such as concentration of hydropropyl-β-cyclodextrin, percentage of methanol, additive of triethylamine in the mobile phase and the velocity of flow, temperature of chromatographic column and et. al. were investigated, the optimum chromatographic conditions of resolution were determined. Secondly, resolution of propranolol hydrochloride enantiomers using β-cyclodextrin and hydropropyl-β-cyclodextrin impregnated thin-layer chromatography was studied synchronously, makeup of the mobile phase and quantity of the impregnated chiral selectors were studied, the conditions of separation were optimized. Thirdly, resolution of clenbuterol hydrochloride enantiomers by thin-layer chromatography using chiral selectors as chiral mobile phase additive or using chiral selectors-impregnated thin-layer chromatography was contrasted, the conclusion was that the ability of resolution of enantiomers using chiral selectors-impregnated thin-layer chromatography was more excellent, the conditions of chiral separation were also studied and optimized.On the study of mechanism of chiral separation, the molecular functionary model of resolution using chiral selectors-impregnated thin-layer chromatography was set up on the view of the action among the molecules. The model of resolution of chiral drugs using (2R,3R)-dialkyl tartrate impregnated thin-layer chromatography was discussed. The conclusion was that the hydrogen bonds formed between (2R,3R)-dialkyl tartrate and silica gel was the main acting force, chiral stationary phase was formed under the acting force, and chiral recognition was then achieved.