Application Of Chiral Crown Ether Stationary Phase In Gas Phase And Liquid Chromatography

Liquid chromatographic separation of enantiomers on chiral stationary phase(CSPs) has been established. The chiral crown ether stationary phase was first developed by Sogah and Cram in 1970 s. The main works of the thesis are listed as follows:R-1,1’-binaphthyl-20-crown 6 was synthesized by(R)-1,1’– bi(2-naphthol), and coated on C18 silica gel(the average particle size 5 μm, aperture 12 nm) as the chiral selectors to separate the racemates in high performance liquid chromatographic. We resolved 5 α-amino acid enantiomers from 21 α-amino acids racemates on the CSP.Using R-(3,3’-diphenyl-1,1’-binaphthyl)-20-crown 6 synthesized by(R)-1,1’– bi(2-naphthol) as the chiral stationary phase for gas chromatography, its molecular recognition ability was studied with enantiomers, geometric isomer, linear alkanes, alcohols and Grob test mixtures.Synthesized R-(3,3’-dibromo-1,1’-binaphthyl)-20-crown-6 and coated it on the C18 silica gel(the average particle size 5 μm, aperture 12 nm) for the liquid chromatographic enantioseparation of α-amino acids, the experimental results showed that the chiral stationary phase possesses a prominent enantioselectivity. Allmost the all tested twenty-one α-amino acids were completely resolved. The separation factor(α) and the resolution(RS) of eleven analytes were better than that of on the commercial crown ether-based column CROWNPAK CR(+).