Curcumin Liposomes And Its Pharmacokinetics

Curcumin has indicated that curcuminoids, the main active ingredient in curcuma, has significantly inhibitory effect on various types of tumors. It can influence each phase of the tumor growth (initiation promotion and progression) and has inhibitory effect on infiltration and metastasis by means of inhibition the tumor angiogenesis. However, curcumin can not be dissolved in water and can also be oxidized easily in vitro, there has been no drug preparation with good stability. Besides, In vivo studies have revealed that small amount of curcumin can be detected in plasma after oral administration . These bring about difficulties in the clinical application of curcumin. Recent studies have shown that liposome encapsulation technique can be employed to solve the problem that fat-soluble drugs can not be easily dissolved in water. Liposome is also an ideal carrier of anti-tumor drugs. It can decrease the toxicity of the encapsulated drugs and increase the amount of drugs absorbed by highly proliferative tumor cells. Therefore, in this study we applied liposome for . the encapsulation of curcumin and evaluated its effects in animal body. The purpose is to exploring the possibility of new preparation of curcumin suitable for venous administration using liposome as a carrier and further understanding the effects of curcumin on the biological properties and to provide proof for the further studies. Methods and results1, The content of Curcumin was determined by Ultraviolet-Visible spectrophotometry and study on the methodology. According to pharmacopoeia(2005), the methods of envelopment ratio was established. 2, Studied the membrane material purchased in the market by oxidizing index method, the result showed that the soybean phosphatide fulfiled the requirement. The liposomes were prepared by the following methods: TFV, REV(reverse-phase evaporation vesicle method),technique of ethanol infusion , aether infusion and hot-melt. The result indicated the encapsulation of liposomes prepared by TFV was the highest. 3 , The best craft produce was investigated by single factor. Experimental factors: Lecithin:Cholesterol was 1:3 and 0.01moL·L^-1 phosphate buffer(pH=6.6) was 20mL. Curcumin was 1mg.4, The curcumin liposomes solution was dried by freeze-dry and chose the best protective agent from fucose, glucose and glycerin. The best one was 15% fucose.5, The quality of Curcumin lipsomes were evaluated.The lipsomes shape was spherical or ellipsoidal, and their size range was 140-425nm by TEM. The surface charge of lipsomes was negative by electrophoresis and...