Pre-column Derivatization Rp-hplc Analysis Of The Method Of Bile Acid Substances

Researched three bile acids in XIONGDAN power, and established a method to detect acids. Bile acid has a lower absorb of UV, Chose proper derivative reagent to react and got chromophores and detected conjugated bile as well as Tauroursodeoxycholic acid (TUDCA) was performed using pyridine-benzoyl chloride for derivative reagent, whereas unconjugated bile acids as well as Ursodeoxycholic acid(UDCA) and Chenodeoxycholic acid(CDCA) were derivate with 2-bromo-4'-nitroacetophenone as derivative reagent.Introduced HPLC and classed analysis of derivative reagent to analyse bile acid have been established. To develope a pre-colum derivatization HPLC method for the .determination of Tauroursodeoxycholic acid, The derivative reagent was Benzoyl chloride in pyridine, wavelength raised to 235 nm. This method was accurate,sensitive. With 2-bromo-4'-nitroacetophenone for derivative reagent. RP-HPLC UV detector. To investigate the analytical method of Ursodeoxycholic acid and Chenodeoxycholic acid.The wavelength raised to 265 nm. This method was accurate, reproducible and simply.