Fluorescent Probes Developed And Its Analytical Application For The Detection Of Reactive Oxygen Species

Various reactive oxygen species (ROS) are generated in the course of biological metabolism, such as superoxide (O2-.), hydrogen peroxide (H2O2), hydroxyl radical (HO·), nitric oxide (NO) and peroxynirte (ONOO-), these ROS play a vital role in physiology. A rapid rise in intracellular oxidant levels under oxidative stress could cause damage to biological molecules and result in various diseases, such as carcinogenesis, inflammation, ischemia-reperfusion injury, and signal transduction. However, the short live and low concentration of ROS mean to the determination difficult, especially the determination in vivo and in situ has no breakthrough.Fluorescence imaging methods are sensitivity, selectivity, ease of use and takes full advantage of benefits provided by confocal laser scanning microscopy (CLSM). CLSM is a type of high-resolution fluorescence microscopy that can give the fluorescence image of cells and tissues, where ROS were detected by fluorescent probe. Fluorescence imaging method challenged the traditional fluorescent method and achieved the detecting of ROS in vivo and in situ.Near-infrared (NIR) light at around 650-900 nm is less absorbed than visible light by biological substances, and can penetrate more deeply into tissues. Moreover, it has the further advantage that autofluorescence is not observed upon NIR excitation. So NIR fluorescent bioimaging is one of the most fascinating fields in chemical biology, offering research challenges and opportunities.Based on the changes in spectrum characters of the fluorescent probes reacting with reactive oxygen species, we have carried out three aspects of investigation:First, piperidine nitric oxide radical spin labeling fluorescent probe cyanine.7.TEMPO was synthesized as a Near-Infrared fluorescent probe for hydroxyl radical. Cy.7.TEMPO is a non-fluorescent compounds, upon treatment with methyl radical that is produced by the reaction between hydroxyl radical and dimethyl sulfoxide (DMSO) quantitatively, and subsequently generates the fluorescent product.Second, a spectrofluorimetric method using 2-styrylbenzothiazoline (Sty.Bzt) as a new fluorescent probe was developed for the determination of superoxide anion...