Leather-like Trichosporon Yeast Rice Grass Hydrolyzate Production Of Microbial Oil

The cordgrass, Spartina anglica is an introduced species that help to stabilise sediments and reduce wave erosion of sea defences. But in recent years, it tends to invade sheltered sand and mudflats, at the upper low marsh level. To explore its ecological and economic values, the bioconversion of partially detoxified acid hydrolysate of Spartina anglica to lipid by Trichosporon cutaneum was studied. And also due to the increase in the price of the petroleum and the environmental concerns about pollution coming from the car gases, biodiesel is becoming a developing area of high concern.Microbial oil is a good resourse of biodiesel oil supply, as it virtually needs no extra farmland while conversion of agricultural residues and other lignocellulosic biomass into lipid. In our research, The hydrolysis of Spartina anglica at 122°C, using four concentrations of sulfuric acid (1%, 2%, 4% and 6%) was investigated with a solid-liquid ratio of 10% (/v). Maximal total reducing sugar of 22.8 g/L was realized with 6% sulfuric acid for 40 min. Moreover, at 140°C using 1% sulfuric acid glucose was 14.2g/L. The optimal fermentation conditions of cell biomass production were as follows: 200 r/min, initial pH 6.0, and an inoculum level of 10% (v/v), 200 mL of medium in 1000-mL Erlenmeyer flask. Moreover, the optimum concentrating factor of hydrolysate was 4.0. Under these conditions, lipid was produced up to 5.9 g/L,lipid content reaches the highest point which was 46.3%. This work provided valuable information for development of an efficient process to produce microbial lipid from Spartina anglica acid hydrolysate.