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Seabuckthorn Extraction Process And Antioxidant Properties

Posted on:2008-12-21Degree:MasterType:Thesis
Country:ChinaCandidate:G B LiuFull Text:PDF
GTID:2191360215467041Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Flavanones in Sea-buckthorn was studied in this paper. Total flavones was extracted with ultrasonic wave method. It compared the productivity of flavones which were extracted with Conventional method to ultrasonic wave method, and demonstrated the best condition of extracting flavones in Sea-buckthorn. The monomer compounds were gained by the purification technology which used Resin, Polyamide column chromatography and high performance liquid chromatogramphy. The monomer compounds which have the strongest antioxidant capacity also was gained by the experiment of antioxidant.The experiment chose the ultrasonic wave to withdraw the time, the ethyl alcohol density, withdraws factor and so on temperature, material ratio, ultrasonic wave power carries on the gradient experiment, Discovers supersonic method withdraws the sea-buckthorn marc total flavanone the optimum condition. The best withdraws condition is 65% ethyl alcohol solution does with the quality score withdraws the medicinal preparation, by 1:20 (g/mL) the material fluid compares under 78℃conditions withdraws time 40min, the ultrasonic wave power is 60%, the sea-buckthorn marc withdraws the total flavanone under this condition rate is 0.4829%.But the convention returns to the conversion of sixteenths to decimal best to withdraw under the condition in it to have to withdraw for 3 hours, the total flavanone rate reaches as high as 0.6911%.The result indicated the ultrasonic wave law withdraws in the sea-buckthorn fruit the total flavanone rate to be inferior to the convention returns to the conversion of sixteenths to decimal to withdraw in the sea-buckthorn fruit the total flavanone rate high, but the ultrasonic wave law has does not destroy the effective component the characteristic, it withdraws the time short, the energy consumption is low may reduce the production cost, enhances the economic efficiency. The adsorption chromatography column of polyamide was also carefully studied in this article, and gained three kinds of flavones. The third crest of the wave can scavenge the superoxide anions that were produced from the self-oxidation reaction of Pyrogallol and when the concentration of the third compound was 100mg/L, the eliminating ratio was 32.2%. From the experiments, We have known the best extracted technology of seabucktborn flavones, and. it was develops and uses this resources to provide the beneficial reference.
Keywords/Search Tags:sea-buckthorn fruit, flavanone, high performance liquid chromatogramphy, chromatogramphic column antioxidant
PDF Full Text Request
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