Atp, Membrane Separation Technology, Polyamide Resin Separation Of Purified Aloe Polysaccharides

At present, there are some disadvantage in the process of aloe polysaccharides production, such as higher energy consumption, pollution of organic solvent, low activity and so on .so in this paper some effective method of aloe polysaccharide production was constructed ,and the constituents of aloe polysaccharides also was analyzed.Firstly, poly(acrylonitrile-acrylamide-styene) was prepared by means of solution polycondensation, IR spectrum of P(AN-AM-St) shows that copolycondensation reaction really happened, structures and properties of copolymer was investigated by DSC,TG..The result shows: ionic cyclization reaction decreased with styrene increased.Secondly, the ultrafiltration membrane of copolymers was prepared with phase inversion method, the membrane surface and cross section was observed by SEM. The many properties of membrane was investigated, su-ch as permeate fluxes, retention, protein adsorb and so on.The results sho-w: water flux of membrane is 26.33ml/cm²*h, retention is 96% for BSA 68000 and is only 34% for dextran T40000; the pH is very important facto-r for water flux and retention of Ultrafiltration membrane, at the isoelectr-ic pH of BSA, the water flux is lowest and the rejection is highest. So the application field of membrane is very big.Thirdly, the separation and purification of aloe polysaccharides was carried out by Poly(acrylonitrile-acrylamide-styene) Ultrafiltration Membrane as permeate pressure (0.1MPa), pH(3-4), the result show that permeat-e solvent of aloe polysaccharides decreased with the permeate time increa-seed in the permeate solution, and the permeate flux also decreased withthe permeate time increased.Fourthly, PEG6000/(NH₄)₂SO₄ aqueous two phase system was chosen for its best phase separation ability, via study of PEG2000/(NH₄)₂, PEG6000/(NH₄)₂SO₄andPEG10000/(NH₄)₂SO₄system,PEG6000/(NH₄)₂SO₄ aqueous two phase system was chosen as the separation system. Many factors were investigated ,such as pH, the weight of NaCl and CuSO₄. The result shows: when m(NH₄)₂SO₄/m(PEG)is 3,the aqueous two phase system have the best ability to form two phase system; with pH increaseed, the recovery of aloe polysaccharide was obvious decreased, but the rec-overy of protein was not changed; when pH of the solution was 2.0, with the weight of NaCl or CuSO₄ was increased, the protein was enriched in the upper phase, so the recovery of protein was a obvious decreased in the down phase, but the recovery of aloe polysaccharide was not changed. So we can confirm the best technical condition; the ratio of m(NH₄)₂SO₄/m (PEG6000) was 3:1; pH of the solution was 2.0; the weight of NaCl w-as 10.5g or the weight of CuSO₄ was 80mg. So the new separation method was constructed between aloe polysaccharides and protein.Lastly, three kinds of aloe polysaccharides were obtained by taking turns of water, NaCl and NaOH as elution solvent with polyamide resins. The polysaccharides was analysed by IR, UV and GC. The content of two kinds of aloe polysaccharides were determined by phenol-sulfuric acidmethod according to the standard of D-mannose. The content of polysaccharides after water eluted is 90.10%, corresponding to 93.28% when NaOH elution used. The polysaccharides after water eluted was still Analysed by GC, xycose, mannose, glucose, galactose as standard monosaccharide. The results show that mannose is mainly monosaccharide in waterelution polysaccharides and only contain a few glucose.