The Brucea Grease Plasmid Preparation And Quality Research

The Brucea Javanica Oil is extracted from Brucea Javanica which is the traditional Chinese medicine.According to pharmaco- investigation, the Brucea Javanica Oil have effect such as anticancer,anti- pathogen,anti- oxidation,anti-gastrelcoma etc. The Brucea Javanica Oil emulsion has been used clinically,but it is instability multiphase dynamics disperse system .Milk drop is easily creaming and rupture when it is heated, frozen and long-term storage. Bioavailability and solo-curative effection of the Brucea Javanica Oil emulsion is low.Because of the toxicity of the Brucea Javanica Oil, the patients have feeling of nauseated and anorexia. These factors limit utilizing of the Brucea Javanica Oil emulsion.In order to improve the effect of antitumor ,decrease the toxicity,make it accumulate in the target place,the Brucea Javanica Oil was enveloped in the liposome. According to investigation, report of Brucea Javanica Oil liposome Preparation isn't seen in the literatures.The film dispersion method was adopted to prepare the Brucea Javanica Oil liposome. As the experimental result, the quality ratio of lecithin and cholesterol was 2∶1, the quality ratio of blank liposome and the Brucea Javanica Oil was2∶1.The envelopment efficiency of the Brucea Javanica Oil liposome was 93.53%.The physico-chemical property and stability of the Brucea Javanica Oil liposome was studied. The Brucea Javanica Oil liposome was small unilamellar and was scattered uniformity. Their mean geometric diameter is 129.7780nm. The appearance, taste, mean diameter,particle diameter distribution, value of electric conductance, value of pH, K value of the Brucea Javanica Oil liposome was studied in the influencing factor experiment, acceleration experiment and reservation experiment. As the experimental result,the Brucea Javanica Oil liposome was stable . Linoleic Acid which is the essential component of Brucea Javanica Oil was used to be the index component.The surplus percentage of Linoleic Acid was evaluated according to the Gas Chromatogram every certain times in the process of laying out experiment.The change of surplus percentage of Linoleic Acid wasn't significant.This phenomenon illustrated that the Brucea Javanica Oil enveloped in the liposomes was stable. In the acute toxicity test, the LD50 of the Brucea Javanica Oil emulsion and the Brucea Javanica Oil liposome were compared. The LD50 of the Brucea Javanica Oil emulsion was 9.16±0.98g/kg . The LD50 of the Brucea Javanica Oil liposome hasn't been got.The results showed that the Brucea Javanica Oil liposome was safe under the dose of 15.3g/kg.These studies indicated that the safety of the Brucea Javanica Oil liposome was increased compared to the Brucea Javanica Oil emulsion.