Spectroscopy Molecular Probe Technology Aromatic Surface Active Agent Toxicity,

Surfactant is a major pollutant in domestic sewage. The effects of it on the ecological environment and human's health have attracted extensive attention. Researches on protein or gene toxic mechanisms of such pollutants in molecular level, and especially the toxicosis and detoxification process of life macromolecules with aromatic surfactant are rarely reported. Therefore, the study on the toxicosis mechanism of such environmental pollutants and the detoxification methods in molecular level can provide valuable references for the prevention of health hazards from environmental pollution.In the first part, we discuss the studying progress of the health hazards from environmental pollution.In the second part, by using the techniques of resonance light scattering (RLS) and absorption spectra, we studied the toxicosis and detoxifcation mechanicsm of anionic surfacetant SDBS and cationic surfactant CTMAB targeted to bovine serum albumin (BSA). Small quantity of CTMAB combines with SDBS-BSA complex to form SDBS-BSA-CTMAB complex and the I_RLS of system enhanced greatly. With the cumulateion of quantity, CTMAB capture SDBS from SDBS—BSA complex by electrostatic attraction and CTMAB—SDBS complex forms, meanwhile BSA automatically frees, which is corresponding to the toxicosis and detoxification processs. Absorption experiment validates that SDBS induces the denaturealization of BSA and CTMAB facilitates the refolding of this protein.In the third part, by using resonance light scattering (RLS) and absorption spectra, we studied the gene—toxicosis and detoxification process of calf thymus ctDNA with cationic surfactants like cetyltrimethyl ammonium (CTAB) and/or anion surfactants like sodium dodecyl benzene sulfonate (SDBS) . Our experimental findings suggest that RLS intensity is enhanced by the long line assembly of cationic surfactant on DNA macromolecules. The interaction between surfactant and DNA is governed by electrostatic attraction together with hydrophobic forces. In the current study, theπ—πattack interaction between the pyridine ring in the cationic surfacetant cetylpyridine bromide (CPB) which ascribed to the gene—toxicosis process and the DNA base was shown to produce a new absorption at a long wavelength, while no such phenomenon was observed for the interaction of CTAB with DNA. In fact, there is no obvious interaction between anion surfacetantd and DNA except weak hydrophobic and elextrostatic repulsion forces. We found that the addition and DNA except weak hydrophobic and electrostatic repulsion forces. We found that the addition of SDBS to the CPB—ctDNA system removed CPB from the CPB—ctNDA system removed CPB from the CPB—ctDNA complex, freeing the ctDNA from the ion—associated complex which is corresponding to the detoxification process. This paper also gives the trend of developments in the field.