Reverse Micelles Cellulase Catalytic Reaction The Best Conditions

Cellulose degradated by enzyme has many merits, such as the relatively mild conditions of reaction, the rather simple equipment of reaction, the low cost of energy and the little pollution. But the large-scale application of enzyme is limited due to the high cost of production of enzyme, the low rate of degradation of enzyme, and so on. Thus reducing the cost of production of enzyme and increasing the efficiency of degradation of enzyme have become the focus of research. At present, most research about degradation of enzyme is done in aqueous solution, but some substrates have higher solubility in non-aqueous solution than in aqueous solution, moreover it can be in more favor of simulating the environment of enzymatic degradation in non-aqueous solution, especially in reverse micelles. So changing the medium of reaction is the very important aspect of studying how to increase the enzymatic activity.In this paper, the power-time curves of the reaction of enzymatic catalysis in reverse micelles were drawn by the TAM Air. According to the thermokinetics theory, the related parameters of systems were calculated. Some important conclusions were also obtained.The main contents are as follows:After determining the concentration of substrate and the doseage of enzyme , the power-time curves of the microcrystalline cellulose degradated by cellulose were separately measured by the TAM Air at different W_o, different acidity(pH) and different temperature(T) in different reverse micelles. Then Using reduced extent method, the apparent Michaelis constant (K_m) and the apparent maximum velocity (V_max) were calculated and the relationships between V_max and W_o , pH, T were established. So we obtained the optimum conditions of microcrystalline cellulose degradated by cellulase.(1) In the reverse micelles of anionic surfactant AOT/isooctane, the optimum W_o is 3.18, the optimum acidity is 5.09 and the optimum temperature is 318.90K;(2) In the reverse micelles of cationic surfactant CTAB/isooctane-chloroform, the optimum W_o is 14.27, the optimum acidity is 4.95 and the optimum temperature is 317.53K;(3) In the reverse micelles of non-ionic surfactant Triton X-100/hexanol-xylene, the optimum W_o is less than 1, the optimum acidity is 5.00 and the optimum temperature is 320.25K.At last, compared with the reaction of enzymatic catalysis in aqueous solution, the enzymatic activity in reverse micelles is higher than that in aqueous solution.The innovation of this paper:Three different surfactants and corresponding solvents are chose to form the systems of reverse micelles in this paper. The use of microcalorimetry is a new studying tool which can accurately and continuously measure the heat changing with the degradation reation of cellulose in reverse micelles. At the same time, the whole reaction process can be seen and plenty of useful information about properties and behaviors of enzyme in reverse micelles is obtained. So microcalorimetric method is a new way to study cellulose degradation by enzyme in reverse micelles.