Preparation Of Molecularly Imprinted Polymer Solid-phase Extraction Performance Study

The dissertation is divided into four chapters, and they are: (1) an overviews of the development of research in molecularly imprinting polymers; (2) an overviews of research of the active component from marine microorganism ; (3) synthesis of MIP nanostructures by using alumina membrane; (4) the synthesis of Molecularly Imprinted Polymers(MIPs) nano-film hybrid silica gel and characters study on it; and (5) discovery of active component of alkaloid from marine microorganism by MIPs.The first chapter is an introduction of the whole dissertation, and it is composed of four parts. The first part is an overview of the development, synthesize route, application and research hotspots of molecularly imprinted polymerization. The second part is an overview of the principle of MIPs nanostructures synthesis using alumina membrane template. The third part is an overview of the principle, influence factors of atom transfer radical polymerization and an introduction of surface-initiated atom transfer radical polymerization. The fourth part is an overview of Reversible Addition-Fragmentation chain Transfer polymerization (RAFT). The fifth part describes the purpose, significant and innovation of the dissertation.In chapter 2, a method for the synthesis of magnetic molecularly imprinted polymer nanowires using a nanoporous alumina template is presented. The magnetic polymer nanowires containing theophylline binding sites uniquely residing at the surface were prepared using alumina membrane as the template. The superparamagnetic nanocrystallites can be well entrapped in the nanowires. The magnetic, molecularly imprinted polymer nanowires developed in this study could have potential applications in drug delivery, drug release, and for trace enrichment of specific target molecules in biochemical application.Chapter 3 describes result on the synthesis and characterisation study of MIPs nano-film hybrid silica gel is described. The MIPs nano-film hybrid silica gel was prepared via Reversible Addition-Fragmentation chain Transfer polymerization (RAFT). The controllable nature of RAFT allows for the growth of MIP nano-film with adjustable thickness on the silica gel surface. Thus, it is possible to tailor the synthesis of either thicker MIPs nano-films for capacity improvement, or thinner MIPs nano-films for efficiency improvement. The results extend the synthetic method of MIPs and the results are significant for the preparation of molecular imprinting solid phase extraction materials and chromatographic media.In chapter 4, the separation and extraction of active substance from marine microbial by MIPs is discussed. MIPs is synthesized in the presence of harmine, a structural analogues of norharman, by RAFT technology. The resulting MIPs was used as a chromatographic media for the separation and extraction of active substances from secondary metabolites of Pseudoalteromonas sp. NJ6-3-1. The result verified the possibility of the separation and extraction of active substance from marine microbial by Molecular Imprinting Technology(MIT). The results of our experiment prove the MIT is a new effective method to discover the active component from marine microorganism selectively.