Synthesis Of Molecularly Imprinted Polymers And Their Applications In Solid-phase Extraction

In this dissertation, molecular imprinting polymers (MIPs) for zalcitabine(ddC), entricitabine(FTC) and lamivudine(3TC) were sythesized in ionic liquids and acetonitrile mixture. The polymers were characterized by swelling experiment, UV, FI-IR and rebinding assay. The obtained MIPs were applied to enrich and separate the anti-HIV drugs in urine by solid-phase extracion.Firstly, the kinds and amounts of ILs were optimized in detail. As a result, [Emim][SCN] was chosed as the assistant solvent and the volume ratio of [Emim][SCN] and acetonitrile was 3:2. The synthesis conditions, incluoding the volume of ILs, the molar ratio of template molecule, functional monomer and different mass fraction of initiator, were investigated in the bulk polymerization by comparing the imprinting factors of MIPs. Herein, the MIPs(NIPs) were polymerized in a thermostated water bath at 60℃for 24h utilizing methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacylate (EGDMA) as cross-linking and azobisisbutyronitrile(AIBN) as initiator with 0.25mmol template molecule and 1:4:20 mol ratio of template molecule, functional monomer and initiator. The imprinting facter of ddC, FTC, 3TC-MIPs were 1.18, 1.13 and 1.28, respectively.Secondly, The MIPs were applied in solid-phase extraction (MISPE). The components of solvent and extraction speed were optimized during loading,washing and eluting steps. Under the optimized SPE conditions, the selectivity of the MIPs (NIPs) was evaluated by extraction the structural analogs of template molecule in acetonitrile. The recoveries of NRTIs on their corresponding MIPs-SPE columns were 97.1%, 96.9% and 97.8% for ddC, FTC and 3TC, respectively. The results also indicated that the more similar the analogues chemical structure is, the higher the recovery is.Finally, the obtained MIPs were directly applied to extract the NRTIs in urine without any pretreatment. The recoveries by loading 200 mL urine sample (containing 20 ng/mL of NRTIs) on MIPs columns were 71.3%, 69.1% and 71.6% for ddC, FTC and 3TC, respectively, while the recoveries of NIPs column were much lower than that of MIPs column.