Study On Preparation And Bioactivity Of New Tea Green Pigment Zinc

China was the largest tea growing area of the country,Tea Green Pigment(TGP) as the by-products in tea processing,had the wide array of sources,the low price and the high comprehensive utilization value.The structure reformation of TGP,not only enhanced the stability,but also made it having the better water-soluble,so that it had the role of colorant and zinc preparations.With the massive planting of tea,the tea wouldl be having a large number of by-products.In this paper,studied the Preparation condition,the Structure,Properties,stability and development of applications on Tea Green Pigment Zinc(TGPZ).And explored the bioabailability and zinc supplement effect of TGPZ.The results was as follows:1.The preparation of the TGP and studied the nature,structure and stability of TGPZ(1) Took the 4,5 to the fresh leaves of tea leaves as raw materials,through the extraction conditions in different contrast,the extraction rate that was:Microwave＞heating＞ultrasonic＞atroom temperature;(2) Detemrmined the content of the amino acid,soluble sugar,tea polyphenol and flavonoids of TGP,and the ability of removed the Free Radical of·OH and O₂-·.In the process of modifying the TGP,The content of tea polyphenols,flavonoids Compounds,amino acids and soluble sugar decreased significantly,especially the content of TP and flavonoids in Tea Green Pigment Zinc was the 32.27 percent and 56.46 percent of the fat-soluble TGP.The reduction of these active ingredients,TGPZ removed the ability of·OH radical compared fat-soluble TGP decrease 66.38 percent.The zinc ions of TGPZ enhance the activity of SOD,so they had the same capacity removal free radical of O₂-·.(3) Through color analysis,The TGPZ was more high and stable than fat-soluble TGP of the value of green,(4) The UV-visible and infrared scans showed that TGPZ compare with fat-soluble TGP the absorption peak of UV-visible moving to the short wavelength,It had a wide peak in 3417 cm^-1 and a strong absorption peak in 996.76 cm^-1 of IR was the logo of substution the metal salts into chlorophyllin. 2.Studied on the bioactivity of Tea Green Pigment Zinc(1) The physiological tested of TGPZ,taking inorganic zinc(ZnSO4) as a reference,the bioavailability of TGPZ was 108.4 percent.And the absorption speed Ka of TGPZ was slower than ZnSO₄,but the absorption peaks was more than ZnSO₄. So TGPZ had a effect of sustained-release and reduced the elimination rate of zinc in the animal.(2) The zinc preparations tested with different doses of TGPZ,showed that SZC2 group had the best effect.TGPZ could promote performance and enhanced immunity of animal.And enhanced the the body's anti-oxidation and increased vitality of AKP;Increased the TC and TG value of serum and spleen organizations and reduced the TC and TG value of the liver.