Natural Indigo Production Process And Quality Control

The Indigo Naturalis is dried powder or agglomerate which is processed by Baphicacanthus cusia (Nees) Bremek.,Polygonum tinctorium Ait. and Isatis indigotica Fort.. In this project, we took a comprehensive, thorough and systemic research on preparing procedure, quality criteria, stability in order to establish the quality criteria with standardization combining the theory of traditional Chinese medicine.The traditional technology is consummated and the quality unstable aspect which the method creates roughly and the traditional technology backwardness is remarkably changed . The craft parameter is determined through the experimental study. The preparing procedure which can comprehensively enhance the output and the quality of the Indigo Naturalis is obtained finally. Fresh stem and leaf of Baphicacanthus cusia (Nees) Bremek.is flushed clean and made into 10cm section, added 20 times water to soak for 48 hours. The soak is filtered and refined through the grit of 10 mu and then through the grit of 40 mu. The 8% lime is added in the filtrate (i.e. 8g lime per 100g stem and leaf of Baphicacanthus cusia (Nees) Bremek.) and then be stirred uniformly. The crude indigo can be get when the liquid is filtered and the filtrate is abandoned. The crude indigo is added the water and made into 2.5% crude indigo pastes and pumped into the levigating automatic sorted machine with the 1500r/min rotational speed to separate the impurity. Then the slurry of the Indigo Naturalis is centrifugated and dehydrated in the 1000r/min rotational speed. The intermediate product is dried in 80℃for 12 hours and pulverized through the grit of 100 mu. This project have established the theoretical and practical foundation for systematized research and the automated and formalization of the Indigo NaturalisThe quality specification has been systemic researched and the quality specification draft of the Indigo Naturalis has been established, we optimized the TLC identification with standards of indigotin and indirubin. The systemic methodology for determining the content of indigotin and indirubin by HPLC has been established and the content of indigotin cannot be lower than 5.30% and the content of indirubin cannot be lower than 0.130%. The preliminary stability test and the acceleration stability test have been carried on.In this project,the preparing procedure and the quality control have been researched, meeting objectively, scientific, controllable, the precise modernized production requirements. The quality specification has been consummated to guarante the effective, safe, controllable, stable quality of production.