Iridium Phosphorescence Synthesis And Its Application In Biological Imaging

Phosphorescent Iridium complexes have received considerable attention due to their high emission efficiencies and easy tuning of emission colors by changing chemical tructures of ligands. In this thesis, an anionic iridium complexe was synthesized which could use for mercury ion detection and the cell marker. Condidering the high relaxation rate, excellent thermal stability and low toxic of Gd ( ) complexes with, we designed and synthesized a water-soluble magnetic-optical dualmodality molecular probes of Ir-Gd heteronuclear metal complexe and explore its application to fluorescence imaging and magnetic resonance imaging in vivo. This thesis can be divided into two parts:1. Phosphorescence anionic Iridium complexes and its application to mercury ion detection and cell markerA bisfunctional anionic phosphorescent iridium complex TBA[Ir(dfppy)2(NCS)2] was synthesized. In the presence of Hg2+, the obvious decrease of luminescence intensity was investigated in details, which could be observed by the naked eye. Due to its strong thiophilic affinity, the special chemical reaction induced by Hg2+ is responsible for the significant change of absorption and luminescence spectra, which is confirmed by ESI-MS. Furthermore, TBA[Ir(dfppy)2(NCS)2] can be a phosphorescent biomarker of the cell membrane.2. Ir-Gd heteronuclear metal complexes synthesis and its application to biological imagingWe have designed and synthesized a heteronuclear metal complexe (Ir-Gd) with iridium phosphorescent complex and rare macrocyclic polyaminepolyacid lanthanide complexe. The cell fluorescence confocal imaging experiments show that the complex can penetrate cell membrane and stained cell cytoplasm region. The tests result from solubility, cell toxicity and magnetic properties shown that the Ir-Gd complexe has good water solubility, lower cell toxicity and higher longitudinal relaxation rate. The data from magnetic resonance imaging contrast agents in vivo shown that the complex can be used for magnet-optical dualmodality molecular probes in vivo.