Fluorescent Probe Molecules For The Marking And Separation Of Amines And Alcohols

Reversed-phase high performance liquid chromatographic method has been widely used in many research and application fields, such as analytical chemistry, biochemistry, clinical medicine, pharmaceutical chemistry, food hygiene and environmental monitoring. It has been one of the fastest developing and the most widely used methods in analytical chemistry due to its powerful separation capacity. However, because of lacking highly sensitive all-purpose detector, researchers adopted chemical derivatization to improve detection sensitivity and baseline separation efficiency. Developing new fluorescent probe molecules and their applications in HPLC were the most active research areas in chromatography. The main idea of this paper was to modify as-synthesized fluorescent cores and to show them broad applications. The modification process, structural determination, spectral properties, derivatization reaction conditions, chromatographic separation conditions, mass spectrometry qualitative and quantitative analysis were investigated. A pre-column derivatization based on reversed-phase high-performance liquid chromatographic method was established and evaluated for the analysis of real samples. The specific work as follows:Chapter one:The history and basic theory about high performance liquid chromatography and high performance liquid chromatography coupled with mass spectrometry were simply introduced. Chemical derivatization, influencing factors of fluorescence intensity, and properties of fluorenscent probe molecules were systematically introduced. Lastly, fluorescent reagents which were used to label amines and alcohols in recent decades were summarized.Chapter two:Pre-column derivatization methods for the sensitive determination of polyamines using DBCEC-C1 as labeling reagent followed by HPLC-MS analysis has been developed. The optimal derivatization was evaluated by using response surface analysis. And the feasibility of this method has been identified by analyzing fish and bean curd with odor samples.Chapter three:The synthesis of the fluorescence labeling reagent 2-((2-methylnaphthalen-1-yl)(1-methy-lnaphthalen-2-yl)amino)ethyl 1H-imidazole-1-carboxylate (DBCEIC) was descr-ibed and followed by the structural characterization and spectral properties. Pre-column derivatization methods for the sensitive determination of fatty amines using DBCEIC as labeling reagent followed by HPLC-MS analysis has been developed. The optimal derivatization and chromatographic separation conditions using DBCEIC as labeling reagent were evaluated. And the feasibility of this method has been identified by analyzing water sample in pharmaceutical factory.Chapter four:The synthesis of the fluorescence labeling reagent 2-(2-(anthracen-9-yl)-1H -benzo[d]imidazol-1-yl)acetic acid(ABIA) was described and followed by the structural characterization and spectral properties. Pre-column derivatization methods for the sensitive determination of fatty amines and fatty alcohols using DBCEIC as labeling reagent followed by HPLC-MS analysis have been developed. The optimal derivatization and chromatographic separation conditions using ABIA as labeling reagent were evaluated. And the feasibility of this method has been identified by analyzing real samples.