Three Separation Media Synthesis And Protein Adsorption Properties

Proteins are bioactive molecules and possess various groups on their surfaces, which can cause interfacial phenomena on the surfaces of various substances. Studies on adsorptive behavior of proteins on a variety of interfaces are of great importance in understanding separation and purification of protein, making it a very active research field. Three kinds of adsorption mediums were prepared in this paper, and the adsorptive properties of protein on them were investigated as well. This thesis is composed of four parts:(1) Review:Interfacial phenomena are encountered widely in the nature. Adsorption at the interface of liquid-solid systems is more complex and more important than that of gas-solid systems. The fundamental conception was briefly introduced in this chapter, including the importance of studying the adsorption in liquid-solid systems, the characteristics of liquid-solid adsorption, the adsorption of macromolecule to liquid-solid interfaces, the types of adsorption isotherms and the adsorptive thermodynamics. Some common adsorbents were reviewed, and the emphasis was placed on the discussion of three novel adsorbent macromolecular materials.(2) In reverse suspension system, an organic-inorganic composite microsphere, poly (AM-co-10%MAA)/CdS, was prepared with poly (AM-co-10%MAA) as the template by exogenous deposition method. The adsorptive behavior of protein on the surface of the composite was investigated using bovine serum albumin (BSA) as the model protein. The results showed that the folded structure of microsphere surface had certain adsorption to the protein tested, preliminarily suggesting the prepared microsphere to be a potential separation material.(3) Molecularly imprinted polymer (MIP) beads imprinted with BSA were prepared via inverse phase suspension polymerization with BSA as the template, both acrylamide and methacrylic acid as functional monomers, N, N'-methylene bisacrylamide as the cross-linker. And the adsorptive behavior of BSA on the surface of the MIP beads was investigated. It was found that the MIP beads prepared under optimized conditions showed specific recognition ability to BSA and the adsorption of BSA fitted Freundlich adsorption model as well as an endothermic process.(4) Poly (glycidyl methacrylate-co-ethylenedimethacrylate) (PGMA) beads were prepared by a single-step swelling and polymerization method with PS beads as the seed. The weak cation exchange (WCX) beads were obtained by hydrolyzing PGMA and then reacting with butanedioic anhydride. The adsorptive behavior of Lys on the surface of the WCX beads was investigated It was found that the adsorption model of Lys on the resulting WCX beads fitted Langmuir adsorption model. The application of the WCX beads in chromatographic separation was carried out. The results revealed that the packing materials could perform well even under high pressure, and basic proteins could be separated in the order which is consistent with the retention rule of cation exchange chromatography.