Analysis Of Microbialcommunity Structure And Dynamics In Egsb Denitrifying Sulfide Removal Process

Now, many researches have proved that organic wastewater could realize the denitrifying sulfide removal, and circled the sulfide by the coordination of autotrophic -doheterotrophic microbial community. For improving the coordination of autotrophic and and doheterotrophic system to remove the sulfide, it is necessary to realize the microbial structure, functional groups and the relationship between microorganism and this process changing, according to the realization, we could control this process. Anaerobic active sludge in EGSB is what this study have researched, utilizing PCR-DGGE, this paper analyzed microbial community structure in different operation conditions to guide the process operating.According to the specialization of anaerobic active sludge in process of coordination of autotrophic–doheterotrophic denitrifying sulfide removal, this paper has optimized PCR-DGGE, DNA extract method, PCR experiment and DGGE drawing condition. The result is successful. What this study has optimized could help analyze microbial community and functional groups.Utilizing the optimized PCR-DGGE, this experiment studied that on the condition of increasing the rate of carbon and nitrogen, sulphide concentration and adding the nitrite, how the autotrophic-doheterotrophic denitrifying sulfide removal microbial community change to ascertain the process condition. The results shows that in the process of increasing the rate of carbon and nitrogen, microbial community structure has not the obvious transformation, on the condition of different rate of carbon and nitrogen, the primary functional microbial community are Sulfurovum and Pseudomonas. In the process of increasing sulfide concentration, Sulfurovum,Pseudomonas is overwhelming, Rhodobacterales Clostridium is minority. Sulfurovum,Thauera,Pseudomonas are the primary functional microorganism which are restricted by the nitrite. Sulfurovum has the function of autotrophic simultaneous denitrification; Thauera and Pseudomonas could utilize acetate as carbon source and nitrate as electron acceptor to proceed along doheterotrophic denitrification. Nitrite in this process can not restrain microorganism.According to analyzing the microbial community structure with PCR-DGGE, in the better autotrophic–doheterotrophic denitrifying sulfide removal, Sulfurovum as autotrophic simultaneous desulfurization and denitrification and Pseudomonas as doheterotrophic denitrification are overwhelming.