Molecular Cloning And Expression Analysis Of Cu/zn Superoxide Dismutase Gene (mrsod1) Cdna From Myrica Rubra

Superoxide dismutase(SOD) is an important enzyme in organism, which can remove superoxide free radical, and it play a central role in protecting cells against the toxic effect of reactive oxygen specices. In this study, the total RNA was extracted from the Myrical rubra leaves. A full-length cDNA sequences of Cu/Zn-SOD whose name is MrSOD1(Genbank accession No. GQ404510) was cloned from Myrical rubra leaves by using the RT-PCR technology. Its length is 461 bp encoding a 152 amino acids Cu/Zn-SOD ortholog. MrSOD1 contains the Cu/Zn-SOD family signature sequence (GFHVHAlGDtT and GNAGgRvACgiI). The analysis of sequence homology showed that MrSOD1 has the homology more than 77% comparing these sequences with other Cu/Zn-SOD genes from GenBank. Protein hydrophobicity analysis showed that MrSOD1 has two obvious transmembrane regions. The expression of MrSOD1 was found in leaf, tress, fruit and flower of Myrica rubra. The expression levels in different tissues from fruit, leaf, flower and tress ranking high to low, with the relative content of 2.8714, 1.1489, 1.0321 and 0.2376, respectively.