Chinese Wild Grape White Rot Disease Resistant, Anthracnose Gene Rapd Markers And Its Clones

The resistance to White Rot & Ripe Rot of the berries of grapes were studied by field natural identification using 168 plants which include 32 clones of 8 Chinese Wild Vitis species, 106 F1 individuals of the crosses between Chinese Wild Vitis and Vvinjfera, 14 individuals of self-pollination cross of Chinese Wild 1tis, and 16 cultivars of Vvin/era. Additionally, the RAPD Markers for the genes of resistant to White Rot & Ripe Rot in Chinese Wild Vitis were tagged. The results were as follows: 1. The resistant phenotygpes to White Rot in 8 Chinese Wild Vitis species, including 32 clones, were both rich and diverse under field natural indentification. Firstly, there existed obvious resistance differences among these species. The resistant species were V davidii, V amurensis, V davidii var. Ninqiangensis, V spp; the susceptible species were V quinquangularis, V Thunbergii. Secondly, the resistances among clones within the same species were different too. Of 13 clones, 9 clones were resistant to White Rot, and they were Liuba-8, Liuba-9 of V piasezkii, Jiangxi-1(-~.), Pingli-2, Pingli-7, Liuba-1 of V romanetii; Baihe-35-l, Baihe-l3-l of V pseudoreticulata, Anlin of V Thunbergii. On the other hand, 4 clones were susceptible to the disease, including Gansu-91 of V piasezkii, Jiangxi-2(~-) of V romanetii, Taishan of V Thunbergii. But foregoing Chinese Wild species were resistant to Ripe Rot under field natural identification, the resistances both species and clones with in the same species weren't different. 2. Based on the characteristic of the segregation and phenotype resistant to White Rot_& Ripe Rot from the progeny and offspring both interspecific hybridization and self-pollination, the researches experimentally suggested the inheritances of Chinese native Wild Vitis species and Clones resistant to White Rot were controlled by major enes, belonging to qualitative traits. In addition, there were minor polygenes in Chinese Wild susceptible Jtis species and Clones. At the same time the researched experimentally suggested the inheritances of Chinese native Wild Vitis species and Clones resistant to Ripe Rot were controlled mainly by major enes, belonging to qualitative Waits, whether there were minor resistant polygenes it is necessary to further research Chinese Wild Vitis species. 3. V vinfera cultivars ordinarily were susceptible to White Rot, But both susceptible 83-4-96( ..) and susceptible Muscat Rose(Chenin Blance) were showed in segregant population from the same cross combination, Enve if the different cross combination were demonstrated the sharp different to the segregation of the disease. So, there were minor-resistant polygenes which enhance the resistant ability in Chinese Wild Jtis species. 4. OPPO9-769 and OPC15-1300, two RAPD Markers for White Rot & Ripe Rot resistant gene of Chinese Wild Vitis were obtained respectively. 5. OPPO9-769 and OPC15-1300 fragments were cut from Agarose electrophoresis gel and cloned in T-vector easy, then sequenced from two sides. The actual length of OPPO9-769 which was sequenced was 769 bp. 5 nd sequence of 516 bp and 3 nd sequence of 516 bp of OPC15-l300 were sequenced from two directions. Because, OPC15-1300 fragments was over l000bp, the total base-sequence of that was obtained by further sub-clone. 6. According to the sequences obtained, two pairs of specific primers including primerbl.. primerb2 and primertl. primert2 were designed to perform PCR. The RAPD Mar...