Compositae Preliminary Study Of The Bactericidal Activity Screening

Compositae is the biggest family of angiospermae,the most complex in components and of the most evolutional family. It is included in 1000 genera, 25000-30000 species. There are about 200 genera distributed in all over the country of China. More attentions are being paid to its insectical components. While little is Known about its fungicidical consitituents. From the July of 1999 to the June of 2001, antifungal activity of the 15 genera 25 species Compositae Plants were collected and studied by writer.The plants acetone extracts was tested by seven fungus, Those are commonly representative and harmful in farming. Such as Botrytis cirerea Pers. et Tris; Gibberella zeae(Schw)Petch; Exserohilum turcicum leonard; Phytophthora capsici leonian; Glomerella cingula Schr; Pseudoperonospora cubensis (Berk.et Curt.)Bostr; Erysiphe graminis DC.f.sp.tritici E.Marshal.The results showed: ①More of the collected plants' acetone extracts were antifungal to more than one tested fungus;② C. macrocephalum Franch. et Sav, I. salsolo deb (Turcz) Ostenf were tested by every part. The results showed C. macrocephalum Franoh. et Sav' s antifungal activity extracts concentrated in its flowers, then leaves, stems and roots. I. salsole deb (Turcz) Ostenf's antifungal activity compounds concentrated in its flowers and leaves;③The same time, 1t was found that C. macrocephalum Franch. et sav's antifangal compounds are concentrated in the acetone and alcohol extracts. They can extract the plants' active components;④The 80℃ was better than the 25℃ when the antifungal activie substances were extracted from the plants, and the tested results are not affected by the temperature. The hot extraction and normal extractive ways both were used by turn;⑤The results showed that the acetone ertract of C.macrocephalum Franch. et sav was the most active one in the tested samples. EC50 were 3280.34μg/ml,3932.28μg/ml,5259.20μg/ml,1278.49μg/ml,100.97μg/ml for inhibiting the growth of the hyphae of Gibberella Zeae(Schw)Petch, Glomerella cingula Schr, Botrytis cirerea Pers, et. Tris, Phyphtora capsici leonian and Exserohilum turcicum leonard.By the bioassay-guided, compounds protested by means of tube-test and TLC. There are flavonids, lactones and coumarin, phytosterols terpenoids, glycosides and so on in the extracts.