The Primary Research Of Antifungal Activity Of Stellera Chamaejasme

To discover novel plant-derived products with pesticidal properties is an important way to create new pest-management agents. It is a obvious resources advantage to research botanical pesticides in western of China for its abundant plant resources. The insecticidal activities of many plants in this area have been studied by Zhang Xing and Wu Wen-jun etc , the antifungal activities of 200 plant species of the area have also been screened by the Research and Development Center of Biorational Pesticides,. In order to utilize the plant resources sufficiently , the antifungal activity of 25 plant species collected in the area have been tested , and the antifungal activity and active ingredients of Stellera chamaejasme selected have also been researched in this paper based on the former works.The results are as following:The antifungal activity of 33 species of plant aceton extracts were tested in vitro with Botrytis cinerea,Fusarium graminerum,Glomerella cingulata,Phytophthora capsici, Exserohilum turcicum etc in the laboratory . Results showed,in the extracts concentration of O.lg/mL, that the extracts of A. gmelinii Web ex Stechm.(leaf)^4.wo玤o/zccr Fisch(stem,leaf),C. canadensis (L.)Cronq(leaf), A. lappa L.(\vhole),S.iodostegia Hance(whole) ,C.pilgeriana(whole) and S. flavescens Ait.(stem) had more than 60% antifungal rates to mycelium growth of two or more tested fungi, S. iodostegia Hance(whole) and S. flavescens Ait. (stem) had stronger effects to 4 species of tested fungi , and the extract of A.mongolica Fisch(stem and leaf) has strong inhibition activity to spore germination of Botrytis cinerea and Fusarium graminerum. The extract of Stellera chamaejasme(iooi) also had stronger activity to mycelium growth of Exserohilum turcicum (Pass.) Leonard.&Suggs., Phytophthora capsici Leoa,and spore germination of Fusarium graminearum Schw^ Exserohilum turcicum (Pass.) Leonard.&Suggs. Glomerella cingulata (Stonem) Schr .et Spauido Those 4 species plants were worth developing.2 The active ingredients of Stellera chamaejasme have been sepatared with bioassay-direction. The powered root of Stellera chamaejasme were extracted with alchol and got the residue after removel of the solvent, the residue was suspended in distilled H2O and was successively partitioned using petroleum ether, chlorin, acetic ether and butanol ; the petroleum ether and chlorin residues were seppatared with chromatograph and TLC etc methods ,and three compounds Ri,R2,R3,were isolated from the petroleum ether residue.3.The structures of RI was identified as P -Amyrin-Ac by spectroscopic menthods.4 The antifungal activity of extracts of Stellera chamaejasme were tested , Resultsshowed, that in the extracts concentration of 0.1g/mL,the extracts of the petroleum ether and chlorin residue had stronger inhibition to mycelium growth of Botrytis cirerea Pers. et Tris, Phytophthora capsici Leon. ,Alternaria solani (Ellis et Martin) and Phyricularia grisea (Cooke) Sacc) , their inhibition rates were more than 75%; in the concentration of 0.08g/mL ,that the extracts of the petroleum ether and chlorin residues had stronger protection effects to Pseudoperonospora cubensis(Berk et Curt.) Rostov and their effects were 76.85%, 64.09% respectively which were more higher than 52.22%-the effect of the alchol extract; five solvent residues had less than 40% therapy effects to Blumeria graminis(DC.)Speer while the petroleum ether residues had 64.9% therapy effects to the fungi.5 The antifungal activity of the compound 3 - Amyrin-Ac were also tested.Results showed in the concentration of lOOOmg/L, the compound had more than 70% inhibitionrats to mycelium growth of Botrytis cirerea Pers. et Tris and Phyricularia grisea (Cooke) Sacc in vitro test; in the concentration of lOOOmg/L, it had 35.2% protction effect to the Pseudoperonospora cubensis(Berk et Curt.) Rostov in vivo test.