| The techniques of SDS-PAGE, IFE-SDS-PAGE, Brilliant Blue and Commassie Dye were used to study the fractions of Vegetative Storage Protein (VSP) and the concentrations of soluble protein in the leaves and branches of Ginkgo biloba from September 2002 to next March. The results were showed:1.Either from a male tree or from a female tree of Ginkgo biloba, the soluble protein concentrations in the leaves began to increase from the middle of September to the end of October. But it began to decrease from the beginning of November, and to the lowest at the middle of November at which all leaves in the trees were yellow and began to fall down. The soluble protein concentrations in the leaves from a male tree were obviously greater than those from a female tree in any period of this experiment.2.Such a temporal tendency of the soluble protein concentration was also observed in the branches from a male tree and a female tree of Ginkgo Biloba. The concentration of the soluble protein increased gradually from Autumn to Winter, reflecting the accumulation of the soluble protein in the branches, while it significantly decreased from December to February, early of Spring, indicating the decomposition of the protein and thus supply of N for the germination of Ginkgo biloba in that period. From February to March, howeve, the concentration of soluble protein in the branches increased again, this might be associated with the fact that the absorption function of roots began to become storage due to an increase of climate temporary and thus more N was obtained from soil at that period. Similart to the leaf, the soluble protein in the male branches was also more than that in the female branches at any periods. The was possible due to the stronger growing potential of the male trees in comparison with the female trees.3.There were 13 kinds of protein observed in the leaves of Ginkgo biloba, including 59kD, 55kD, 50kD, 40kD, 38kD, 32kD, 28kD, 27kD, 22kD, 19kD, 18kD, 17kD, 16kD. In which only 55kD and 32kD protein were detected to vary with time in concentration by the technique of SDS-PAGE and IEF-SDS-PAGE, but 18kD was only detected by IEF-SDS-PAGE.For the protein 55kD, its content in the leaves from a male tree began to vary at the middle of October, earlier than from female tree at the beginning of November.For the protein 32kD, its content, either in the male leaves or in the female leaves, varied at the beginning of November, and such the range of variation over time in the female leaves was obviously greater than in the male leaves, indicating that protein 32kD showed be an important VSP of female tree.As to the protein 18kD, they began to transfer from the beginning of November, they were difficult to find its spots in the electrophoregram of leaves from the middle of November. And this variation of the protein bands or spots were only detected by the IEF-SDS-PAGE.Consequently, the changes of VSP in the leaves were generally taken place in November, especially in the middle of November at which all leaves became yellow and began to fall down.4. In the branches, 14 kinds of protein were observed, such as 59kD, 54kD, 45kD, 40kD, 38kD, 35kD, 33kD, 32kD, 30kD, 29kD, 23kD, 18kD, 17kD, and 16kD. The protein 59kD, 32kD, and 27kD detected by SDS-PAGE and IFE-SDS-PAGE, and 40kD, 38kD, 22 kD, 18kD and 16kD detected by IEF-SDS-PAGE varied with time. And such the variations of protein content were different in the stage of accumulation and decomposition.In the stage of accumulation:Protein 59kD: Based on the electrophoregram, it began to accumulate at the beginning of November and then gradually increased through November. This accumulation reached its top at the end of December for the female branches.Protein 40kD and 38kD: They were found by the electrophoretic pattern of IEF-SDS-PAGE that the contents of this two kinds proteins were higher in the male branches than in the female branches, and each of them began to increase at the November and reached its top at the end of December. However, such a trea... |
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