Snowdrop Lectin Gene Into Chrysanthemums And Transgenic Plants Aphid Resistance Study

Agrobacterium-mediated chrysanthemum transformation was studied in this paper. Snowdrop lectin gene was successfully transferred into chrysanthemum. Factors influencing transformation were discussed and optimized. Aphid (Mizus persicae) inhibitory effect in transgenic plants was discussed. 93 transformed plants were obtained. The highest transformation frequency was 11.21%. Results from PCR and FQ-PCR analysis confirmed that GNA gene were integrated into the genome of transformed chrysanthemum plants. The results from insect bioassay with aphid showed that the transgenic plants were aphid resistant. The average inhibition rate of aphid population development of detached leaves and whole plants were 38.6% and 39.4%. The experiment of Agrobacterium-mediated chrysanthemum leaves transformation showed that many factors influenced transformation frequency. The results showed that the conditions of Agrobacterium and explants were important. High transformation frequency could be received when Agrobacterium were re-suspended in activation medium (YEB-1) with concentration of OD600 = 0.4. It was important to pre-culture explants before infection, and 1 day was the best. Big explants excised from 45 day-old tube-plants were benefit for obtaining transgenic plants. 5 min for infection time, 4 days for co-cultivation period and temperature of co-cultivation with 26℃-28℃ were the best transformation condition. The transformation frequency was increased from 6.89% to 11.21%. Aphid-resistance of transgenic plants and clotting actives indicated GNA gene was expressed .