Fluorosis Of Silkworm Larvae Of Glutathione (gsh) Metabolism

The fluoride poisoning is serious problem to silkworm cocoon production in main sericultural area of China. In order to find out mechanism of fluoride toxicology to silkworms, the glutathione and some correlate enzymes in hemolymph of the fluoride poisoned silkworm larvae were assayed, and the effects of fluoride on glutathione metabolism of poisoning silkworms were discussed.The experiment showed, the content of glutathione decreased from the first day to the fourth day, while increase from the fourth day to the sixth day in normal larvae. After feeding with NaF solution, the content was degressive, the higher concentration of NaF solution, the lower of the glutathione content. The GST activity ascend from the first day to the third day, while descend from the third day. After feeding with NaF solution, GST activity increased. The y-GT activity ascend from the first day to the fourth day , while flown from the fourth day to the seventh day. After feeding with NaF solution, y-GT activity increased obviously, the higher dose of NaF solution, the higher of its activity. The glutathione synthetases activity always increased from the first day to the sixth day, and after feeding with NaF solution, the activity increased. The GSH-Px activity increased from the first day to the third day, while decreased from the third day to the sixth day, and after feeding with NaF solution, GSH-Px activity increased.The content of oxygen free radical increased from the first day to the third day, while descend from the third day to the sixth day. The content increased after feeding with NaF solution, while the levels of oxygen free radical decreased after GSH treatment in hemolymph of the fluoride poisoned silkworm. The content of malondialdehyde increased from the first day to the third day, after that it presented stable. The content of malondialdehyde increased after feeding with NaF solution, while the levels decreased after GSH treatment in hemolymph of the fluoride poisoned silkworm. The oxygen free radical and malondialdehyde also showed that GSH had an effect of remitting fluoride poison to silkworm.The results implied that possible effects of fluoride toxicology on GSH metabolism were through the regulation of GSH-relative enzyme activities.