Effect Of Fluoride On Glutathione Antioxidant System In Mice Testis And Epididymis

[Objective]In recent years,a large number of studies have shown that fluorosis can cause damage to the reproductive system of male animals,especially to the body's antioxidant system.Oxidative stress is one of the mechanisms of fluoride poisoning.Studying the effect of fluoride on the testis and epididymis of glutathione antioxidant system,which lays the theoretical foundation for further elucidation of the reproductive toxicity of fluorine and the relationship between oxidative stress and fluorosis.[Method]100 three weeks old ICR male mice were randomly divided into control group and fluoride group,each group of 25.The four groups were orally administrated with 0,25,50,and 100 mg/L NaF for 60 d.The growth state of the mice was observed dynamically during the period of fluoride exposure and the related data were recorded accurately.After the end of the exposure,testis and epididymis were collected,the quality of semen,the activity of glutathione antioxidant system,the ultrastructure of testis and epididymis,the expression of glutathione antioxidant system gene and its protein were analyzed,to study the effects of fluoride on the structure and function of testis and epididymis.[Results]1.Compared with the control group,the quality of semen in the fluoride group decreased.The sperm density and viability of the 100 mg/L NaF group decreased significantly(P<0.01),and the deformity rate increased significantly(P<0.01).2.Compared with the control group,100mg/LNaF group of testis:the activities of T-AOC,GPx,GSTs and GR decreased significantly(P<0.05),the content of GSH decreased significantly(P<0.01),and the content of MDA increased significantly(P<0.05);Compared with the control group,100mg/LNaF group of epididymis:the activities of T-AOC,GPx,GSTs and GR decreased significantly(P<0.05),the content of GSH decreased significantly(P<0.01),and the content of MDA increased significantly(P<0.01).3.Transmission electron microscopy was used to observe the ultrastructure of testis and epididymis in control group and fluoride group.Compared with the control group,the testis and epididymis of mitochondria,rough endoplasmic reticulum and Golgi apparatus were damaged at different degrees.4.Real-time PCR resultsicompared with the control group,100 mg/LNaF group of testis:the gene expression of GPx1 was significantly decreased(P<0.01),and the gene expression of GPx4,GST-mu5 and GR decreased significantly(P<0.05);Compared with the control group,100 mg/LNaF group of epididymis:the expression of GPxl,GPx5 and GST-mu5 genes decreased significantly(P<0.01),and the expression of GPx4 and GR genes decreased significantly(P<0.05).5.ELIS A results showed that:compared with the control group,the protein expression of GPx4 and GR expression in 100 mg/LNaF group of the testis and epididymis were significantly decreased(P<0.05);the protein expression of GST-mu5 in fluoride group of the testis and epididymis were significantly increased(P<0.01);the protein expression of GPxl in 100mg/LNaF group of testicular was decreased significantly(P<0.01),the protein expression of GPx5 in fluoride group of epididymis was decreased significantly(P<0.01).[Conclusion]The results showed that fluoride can reduce sperm quality in mice,reduce the antioxidant capacity of the testis and epididymis,causing the oxidative stress in the body.