Napus A New Cytoplasmic Male Sterile Line, The Observation Of Fertility And Strong Heterosis Screening

In newly combination of hybridizing rape F₁ colony of two system of recessiveness nuclear sterile discovery 5 male sterility trunk in Spring of 2004, Through 6 years 9 generations'select, and were cultivated Newly cytoplasm male sterility line 3231A and corresponding maintainer 3231B (They are respectively named RM3231Aand RM3231B), The sterile rate in this colony is 100%, It is not to have the flower bud dying and closes the flower bud phenomenon in florescence, The sterility is more stable , The sterile degree is more than 97%; There is about the breed ( Xi) of 78.7% which is restore lines in the brassica napuso In order to explore the sterile system minute powder's circumstances and application foreground, The thesis is research with RM3231A, We should place the sterile system in different districts, different seasons and sow the observation fertility by stages, and wiht many wild cabbage moulds restore lines hybridize, in order to select the most excellent strong combination, the result of the studying is indicates:1, In blossom later period of RM3231 A's cytoplasm male sterility , there are different level minute powder occurs, it is chiefly distribute in the top of inflorescence, On the same plant, The sequence is : Main inflorescence >the frist divide branch >the second divide branches;2, Bfore the blossom about 20 days , The most temperature is more than about 20 °Cs, The equal temperature of a day is more than 14.5 °Cs, It is the major reason to produce minute powder, The Sterile lines is belong to low temperature sterile type., Under the autumn sowing condition, the sterile degree had descends trend, which along with deferent period of sowing;3, Under the spring sowing and the summer sowing condition, The sterile degree descends than the autumn sowing by a wide margin;4, Asa result of 7 times trial (78 new combinations)in 2000-2004 years, Selecting 5 protruding combinations, They are respectively RM3231A × 6215F₁, RM3231A×6166F₁, RM3231A × MR-3F₁, RM3231A × 10108F₁ and RM3231A× 5785F₁, The common characteristic of these combinations is High yield , Yellow seed , these exellent properties are the production needs;5, In mentioned above 5 combinations, As a result of the trial and the character's analyse and identify, Sending RM3231A × 5785F₁ respectively into the lower reaches ofChangjiang River in autumn in 2003, It is Show fine(ly) in the trial, In 2004, it is entering continuing tries, to produce the trial at the same time, Recommending the RM3231A × 6215F₁ into rape area of Hunan Province province to prepare the trial in 2004.