| Powdery mildew, caused by Erysiphe graminis DC.f.sp.tritici, is one of the most important wheat diseases in China and in many regions of the world. In recent years, the damage of the wheat powdery mildew gets more and more serious in many wheat regions of our country and it made severe damage in wheat production. Though chemistry controlling is useful, breeding for resistance has proved to be the most economical and effective way for controlling wheat diseases. Up to now, more than 30 genes loci for resistance to powdery mildew have been identified. Furthermore, many resistance genes have been tagged and assigned to specific chromosomes or chromosome arms. Among these genes, the Pm21 (6AL/6VS), originating from Haynaldia villosa, is one of the most effective resistance genes.Molecular marker technology is an effective measure for the identification and combinations of the resistance genes and it has been widely used to marker-assisted selection and gene pyramiding in wheat resistance breeding. In this study, amplified fragments length polymorphism (AFLP) analysis was carried out in common wheat (AABBDD), Haynaldia villosa, 6A/6V substitution line and 6AL/6VS translocation line. The objectives of this study were to search new AFLP markers tightly linked to Pm21 gene. It will be also used for the cloning of Pm21 gene and marker-assisted selection (MAS) and wheat resistance breeding.F2 population from a cross between Jing411 and 6A/6V substitution line were identified for resistance to powdery mildew. The results showed the expected Mendelian ratio of 3:1 and indicated that the resistance was controlled by a single dominant gene.With the research of main condition including concentration of template, Mg2+ and primer, the result of pre-amplified is good when the content of template is 500ng, Mg2+ final concentration is 1.5mM and the content of primer is 30ng in 20μl reaction system. Optimal AFLP reaction system is established and it provided advantaged condition for next study. |
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