Study On The Effect Of Haynaldia Villosa V Chromosome On The Proteome Of Wheat-H. Villosa Chromosome Substitution Line And Translocation Line And Its Relationship With The Powdery Mildew

Wheat-Haynaldia villosa chromosome substitution line (6A/6V) and translocation lines (6DL/6VS) were obtained through hybridization of H. villosa with powdery mildew susceptible cultivated wheat. Substitution line and translocation lines contain V chromosome or the chromosome short arm (VS) of H. villosa. They are resistant to powdery mildew. With 2D-PAGE techniques, comparison of leaf protein and root protein in cultivar wheat (AABBDD). Wheat-Haynaldia villosa chromosome substitution line (6A/6V), translocation lines (6DL/6VS) and H. villosa (VV) have been made in this study. Over 350 protein species were detected in the leaf and root of cultivar wheat, chromosome substitution line and translocation line. Their molecular weight ranged from 10-110 kDa and pI from 4.5-8.6. 2D-PAGE patterns of leaf and root protein are almost the same among cultivarwheat, 6A/6V, 6DL/6VS, but different from H. villosa. A specific protein (68kDa/PI7.2) was found in substitution line, translocation line and H. villosa, but not found in the cultivar wheat. This result suggested that the protein (68kDa/PI7.2) may be located in the short arm of V chromosome of H. villosa. In this study, mitochondrial proteome changes were analyzed by using substitution line (6A/6V), translocation line (6DL/6VS) as experimental materials in order to studying the effects of V chromosome on the mitochondrial proteome and related to powdery mildew resistance. The results indicated that 16 new mitochondrial protein spots (22kDa/PI8.5, 31kDa/PI7.5, 28kDa/PI7.0, 31kDa/PI6.5, 40kDa/PI7.5, 40kDa/PI7.4, 80kDa/PI8.4, 50kDa/PI7.5, 60kDa/PI7.3, 65kDa/PI6.6, 65kDa/PI6.6, 73kDa/PI7.5, 73kDa/PI7.7, 46kDa/PI7.4, 46kDa/PI7.3 , 38kDa/PI6.3) were produced and 7 mitochondrial protein spots (40kDa/PI7.5, 43kDa/PI7.6, 48kDa/PI7.5, 42kDa/PI8.0, 43kDa/PI7.5, 32kDa/PI4.8, 40kDa/PI5.5) were absent in substitution line, 7 new mitochondrial protein spots (43kDa/PI6.3, 60kDa/PI6.5, 60kDa/PI6.4, 65kDa/PI7.5, 55kDa/PI8.2, 31kDa/PI8.0, 43kDa/PI8.0) were produced and 6 mitochondrial protein spots (66kDa/PI8.3, 58kDa/PI8.5, 36kDa/PI7.0, 48kDa/PI7.7, 48kDa/PI6.8, 43kDa/PI6.2) were absent in translocation line. These experimental results suggest that V chromosome or VS of H. villosa can obviously lead mitochondrial proteome changed. These changes may be associated with resistant to powdery mildew of substitution line and translocation line.