Rye Genome Specific PCR Marker To Establish Its Wheat - Africa Rye 1RS/2BL Chromosome Translocation Lines Obtained

As the most important food crop world wide, common wheat (Triticum aestivum L.) plays a key role on developing economy, sustaining social stability and prospective in China, which stands the second only after rice. However, the narrow genetic basis of wheat has limited the further improvement of yield and quality, which is essential to maintain the increasing population. Fortunately, a great deal of genetic variation existing in the related species of wheat provided large useful resource for meeting the needs of the modern wheat breeding. Rye (Secale cereale), as the most successfully utilized alien diploid gene donor for wheat, contained a large number of useful target genes including high yield, extensive rusts and powdery mildew resistance. The importance has been widely recognized of engineering the novel rye chromatin to wheat chromosomes for the breeding purposes. Many methods have been produced and used in detection of rye chromatin introgression in wheat, including the morphological, cytological and molecular markers. In this study, based on the high repeat sequences of rye chromatin, new specific PCR molecular marker was developed, and was utilized to detect or track transfer of genes from the relatives of common wheat. In addition, acid polyacrlamide gel electrophoresis (APAGE) and Fluorescence in Situ Hybridization (FISH) were carried out in this paper. Results are shown as follows:1. Based on the sequences of high repeats Psc20H (Genbank number: AF305943), we developed a new pair of primer, and successfully carried out to trace the rye chromatin by PCR analysis. A target bands about 1400bp were observed in cultivated rye and wild Secale species, as well as the known wheat-rye addition lines and 1RS/1BL translocation lines. Among the newly produced advance stripe rust resistant lines derived from Secale africanum, BC2F4 lines L1, L8-L11, L13, L15 and L16 exhibited the specific...