Guizhou Purity Test Of Hybrid Corn And Its Fingerprints Of The Building

Core primers were defined and multiplex PCR was studied by SSR marker. At the same time, DNA fingerprints of maize hybrids and their parents were established. This study was reference for "research on establishing DNA fingerprinting database of xishan maize hybrids and their parents" and the purity and reality identification of maize cultivars. The results were summarized as follows:1. Morphology character of Xishan maize hybrids and their parents was observed in different growth period , and the purity of maize hybrids could be effectively distinguished according to the synthesis of the morphology target.2. 19 maize inbred lines were analyzed using SSR technics. Twenty-three pairs out of one hundred and twenty-three pairs of SSR primers were selected, which gave polymorphic, stable and repeated amplification profiles. 6 polymorphic bands could be obtained from one inbred lines on average with twenty-three pairs primers. All 19 inbred lines were distinguished with the DNA fingerprint datebase which established by 23 pairs SSR primer.3. DNA fingerprint map of maize hybrids and their parents with 27 SSR primers. Twenty-seven pairs out of one hundred and twenty-seven pairs of SSR primers were selected, which gave polymorphic, stable and repeated amplification profiles. 8.37 polymorphic bands could be obtained from one inbred lines on average with twenty-seven pairs primers. The complement type of two parents was the optimal one for distinguishing hybrids and their inbred lines.