Avian Influenza H5n1 Virus Molecular Evolution Analysis And Detection Methods

Avian influenza virus (AIV), one kind of the pathogens that pose significant challenges to both human and animal health for a long time, can cause epidemics and pandemics in many species of birds and mammals with acute respiratory syndromes. Especially the highly pathogenic avian influenza A subtype H5N1 virus, has caused a few human infectors to death. However, the numerous serotypes and the frequent mutation character of AIV, make the surveillance and control more difficult, there still need quick, special, and easy-to-operate methods for detection. So, enhanced research on the evolution and detection of H5N1 AIV is of significant implications in reality.In this paper, we make a research on the evolutions of complete genomes and the individual genes of 149 AIV subtype H5N1, taking use of the free data in NCBI influenza resource and the software of bioinformatics, A large datasets are also comprised, which include the eight genes of incomplete genome to make a correct conclusion, Our aim is to make a further analysis on the mutation features of AIV, and predict its future patterns of evolution for vaccine strain selection. It shows that: first, these H5N1 AIV have different ancestors, each pandemics is caused by the new strains, second, besides of the antigenic drift, the reassortment, which is commonplace and complex, is mostly responsible for the AIV evolution, third, migratory birds play a important role in introducing AIV to other regions. Therefore, enhanced research on the evolution and molecular biology of AIV still needed, and it's necessary to do complete analysis on the AIV genome. It also highlights the necessity of continually updating the candidate virus chosen for future H5N1 vaccines, and requires the intensive surveillance with the global efforts.Furthermore, on the bases of the evolutionary research, we also make an attempt on the detection of H5N1 AIV. A reverse dot blot technique, which can accomplish the detection with the ordinary lab equipment, is preliminarily established.