Different Source Frozen Sperm Induced Turbot And Atlantic Flounder Gynogenetic

1. The sperms of the weever(Lateolabrax japoniaus), whether irradiated by UV or not, both could stimulate the turbot(Scophthalmus maximus) eggs to start the development process. And, the embryonic development of the two groups was entirely the same. Put the eggs into (13±0.2)℃seawater, they will hatch after about 95 hours. Because the genome was not doubled , both of them exhibited aberrant development, being as same as"haploid syndrome". Diploid was induced by activating egg development with Lateolabrax japoniaus sperm for 6.5 min in seawater, and then subjecting the eggs to cold shock in -1~0℃seawater for 25 min. The haploid ones would die out after 5-7 days and the left ones are turbot gynogenesis diploid. There is no difference between the gynogenesis diploid and the common diploid and no crossbreed individuals are found. By comparing the growth rates and morphological characteristics of common diploid, haploid and manual induced gynogenesis diploid embryos, it indicated that there are significant differences of embryos growth rate among them. The rate of common diploid and haploid is larger than that of manual induced gynogenesis diploid. In morphological characteristics, the haploid embryos exhibit a typical haploid syndrome with the characteristics of small head, short tail, weak activation and difficult in breaking membrane. However, the manual induced gynogenesis diploid embryos are consistent with the common diploid in the same term characteristics. It can be conclude that the method used in this study that turbot gynogenesis is induced by sperm of weever has a effective function in restrain the rate of cleavage.2. Paralichthys dentatus diploid gynogenesis was induced by activating egg development with UV irradiated Lateolabrax Japoniaus sperms (80μJ/cm2) for 5 min in seawater, and then subjecting the eggs to cold shock in 3℃seawater for 30 min. The hybrid could not survive to hatching, thus the offspring was diploid only and the result of chromosome analysis proved this result. The number of the diploid gynogenesis chromosome is 2n=48. And the number of the haploid chromosome is n=24.