| Factors influencing sperm motility are various.Aquaporin and p H are important influencing factors.Aquaporin is a kind of integral membrane proteins located in cell membrances.It is essential to the nomal physiological activities of the organism because it modulates hydrone and other small molecules influx or efflux.Up to now,13 kinds of aquaporin was found inmammalia and 8 kinds of them was found in fish reproductive system.The study showed that AQP7 can provide energy by glycerin transportation whlie has no function of water transprotation for sperm.AQP8 is important in regulating sperm volume and the transportation of ROS in miochondrial.Factors influencing sperm motility are various,and p H is one of the factors.In regred to the influence of p H on fish sperm vitality,the previous studies focuse on the influence of different p H of extermal environment on sperm motility,but there are seldom research focuse on the influence of different p H of sperm itself on sperm motility.Turbot(Scophthalmus maximus)is one of the most important fish in the china sea.This paper seting turbot as a study focus,aims to clarify the influence of AQP7 and AQP8 on sperm motility and the expression characteristics in the process of sperm development.At the same time,study the relationship between sperm motility and pH by the detection and evaluation of sperm motility.The main results were as follows:1)Get the full-length gene of AQP7 and part gene fragment of AQP8,1513 bp and584bp respectively.The expression of AQP7 and AQP8 mRNA increased at first and then decreased from stage 2 to 5.The highest peak is stage 3 and stage4 respectively but the expression of both of them increased in stage 6.The results of situ hybridization of AQP7 and AQP8 are consistent with the expression of AQP7 and AQP8 mRNA.AQP7 was expressed from spermatogonia to spermatozoa.It was highly expressed in high quality sperm,which suggest AQP7 has a promotion to sperm viability.2)The sperm with higher motility and longer life p H has a weak alkaline p H(p H≈ 8.0)which means turbot sperm are active in the weak alkaline environment.We found p H of turbot sperm were in significantly positive correlation with motility,VCL,VSL,VAP,ALH,BCF(p < 0.05),while there was no significant correlation with LIN,WOB,STR.The motility of turbot sperm were in significantly positive correlation with VCL,VSL,VAP,ALH,BCF(p < 0.05),while there was no significant correlation with LIN,WOB,STR.3)In addition,we found appropriate amount of glycerol in sea water was useful for maintaining longevity,and the optimal concentration of glycerol was 100mmol/L. |
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