A Broad Spectrum Of Antagonistic Bacteria Wb3 Laser Mutagenesis And Composite Bacterial Manure, Biological Control Applications

Purpose:To identify the genus of WB3 and affirm its antagonistic range.Induce WB3 by laser in order to pick out the mutation strain whoes antagonistic ability has improved mostly, and who can inherit stably.Do mixed culture with WB3 and another broad-spectrum antagonistic B.subtilis QM3,then match the mixed fermentation broth with biochemical fulvic acid(BFA) bacteria fertilizer together to form a new product,multiple bacteria fertilizer,which would be applied in field experiments.Method:Identify WB3 through the way of morphology observation,physio-biochemical detection and 16SrRNA sequence analysis.Affirm the strain's antagonistic range initially by doing flat plate(FP) inhibition tests of WB3 on 12 common fungous diseases.Use He-Ne laser to irradiate WB3 who has a broad-spectrum antagonistic range,during the course,making the Alternaria solani as an indication,hoping one mutation strain whose antagonistic ability can be improved a lot and could inherit stably would be obtained.Try to cultivate WB3 and QM3 together through plate crossing streaking,fluid shake flask cultivation,observing the total number of the bacteria and the variation of the antagonistic ability after the mixed fermentation.Furthermore,try to fumble out a fermentation culture fit for the mixed strains basing on corn flour fluid culture.In the end,match the mixed antagonistic bacteria liquor and BFA bacteria fertilizer to form the multiple biological controlling bacteria fertilizer in order to be used in field experiments and application.Result:WB3 belongs to Bucillus subtilis after the identification,which has an antagonistic ability against the 12 plant pathomycetes.30minutes He-Ne laser irradiation has an apparent mutagenesis effect on WB3.The best mutation strain WB3',whose antagonistic ability against the indication has been improved obviously and could inherit stably.WB3' and QM3 could get with each other well in the same living environment,and their antagonistic abilities can be improved and complement each other.Comparing to the chemical pesticide,multiple bacteria fertilizer has overall advantages in many fields,including disease preventing,curing, and growth promoting.Conclusion:Using He-Ne laser to irradiate WB3 could improve the antagonistic ability of broad-spectrum antagonistic B.subtilis WB3,which can grow with QM3 together in the same nutrition environment.At the same time,there is a promoting effect both in the growing part and inhibition part.Multiple bacteria fertilizer has a satisfactory prevention effect in the field application experiments as well as its manurial effect.