Rapd And Aflp Molecular Markers For Genetic Analysis Of The Cucumber Orange Core

The cucumber tangerine core variety is Heilongjiang Province Institute of Agricultural Sciences Gardening Branch studies the discovery one kind of cucumber new variety,This variety cucumber core color is a red-orange,Compares with the ordinary cucumber variety,The feeling in the mouth is extremely sweet and crisp。This research take Cucumber tangerine core new variety and Cucumber ordinary core variety as a material,Uses RAPD and the AFLP mark technology the tangerine core variety,Carries on the analysis to the cucumber tangerine core gene。Meanwhile conducts the heredity contrast research through the field adult plant character,To RAPD and the AFLP mark which obtains carries on DNA to measure the foreword。In RAPD mark analysis, In screening 200 along with machine-drawn in, Found 1 the polymorphism fragment which directs thing S5160-589 to expand increases,After confirmation,This mark and the tangerine core gene exists altogether grows the relations。Directs the combination using the AFLP mark technology to 250 pairs to carry on PCR to expand increases screening。In which directs the thing the polymorphism fragment which expands to P28M60-56 increases to confirm in during two varieties indicated,This mark and the tangerine core gene exists altogether grows the relations。To RAPD and the AFLP mark which obtains carries on the gene clone and measures the foreword, Thus obtains the DNA mark fragment which altogether grows with the cucumber tangerine core character gene. Unifies through RAPD and the AFLP mark, Meanwhile through field adult plant heredity confirmation, Has discovered the cucumber tangerine core mark for the simple gene heredity recessive heredity.