Tianfu Meat Duck Cavity Sac Of Embryos And Embryo Growth And Development And Expression Of Neuropeptide Research

By the methods of routine anatomic,morphology,transmission electron microscope and immunhistochemistry,the structure of the bursa of fabricius in Tianfu duck and the distribution of five neuropeptides in the bursa were studied during the embryonic and post embryonic development.The developmental pattern,the expression characteristics and physiological function of neuropeptides in the bursa were investigated,which provided theoretical basis for fundamental research,production practice and investigate nervous-immune-endocrine net.The post embryonic development of bursa can be divided into three periods:the continued growth period(0-8weeks),the maturated durative period(8-14weeks) and the degraded period(17-32weeks).The functional relation of the bursal weight and the time were taken on the power function at embryonic stage and the sigmoid curve at 1-14 weeks after hatching.The functional relation of the bursal index and the time were taken on the quadratic function at embryonic stage while the cubic function at 1-32 weeks after hatching.The relationship between the bursal weight and body weight was showed itself to the cubic model at embryonic stage.The histological structure of the bursa was well-developed at 26 days embryonic stage.The emergence and disappearance of the bud break was an important stage during the bursal nodule development.The nuclei of apoptotic lymphocytes were obviously changed during the embryonic and post embryonic development.The positive reaction of calcitonin gene-relatedpeptide(CGRP) originally appeared in the bursal epithelium at 20 days embryonic stage and occurred in follicle at 0-14 weeks after hatching.The smooth muscle layer and arteriolar smooth muscle reacted positively.The bursa showed CGRP negative reaction.Vasoactive intestinal peptide(VIP) positive cells were found in the follicle-associated epithelium(FAE) and follicle from 20 days embryonic stage to 32 weeks after hatching.Interfollicle epithelium(IFE) also showed positive reaction at that time except 1-14 weeks.The smooth muscle layer and arteriolar smooth muscle reacted positively.The VIP positive fibers were located in lamina propria from 5 to 29 weeks after hatching.IFE showed negative reaction of neuropeptide Y(NPY) in each group.FAE and follicle showed NPY positive reaction from 0 to 29 weeks after hatching.The smooth muscle layer and arteriolar smooth muscle reacted positively from 20 days to 32 weeks.Somatostatin(SS) positive cells were observed in the IFE and follicle medulla at the degraded period.The positive reaction particle of brown color was big.Gastrin(GAS) positive cells were detected in FAE from 22 days embryonic stage to 26 weeks after hatching,in IFE from 22 days to 3 weeks.However,the GAS-positive expression of follicle appeared in different time.The bursa of Tianfu duck showed the characteristics of age-related changes during the embryonic and post embryonic development.The positive cells of CGRP,VIP,NPY, SS and GAS were widely distributed in the Tianfu duck bursa at different time,whose number showed the characteristics of age-related changes.The duck bursa was an important source of CGRP,VIP,NPY,SS and GAS,besides the nervous system.