Tibetan Naked Barley ¦Â-glucan And Dietary Fiber Content Of The Genetic And Environmental Effects

Over 60 strains of barleys were collected from Tibet in the experiment, whoseβ-glucan content was determined and analysised by method of Congored stained and whose dietary fibre content was determined by method of cold neutral detergent fibre, effect factors of climatic factors on content ofβ-glucans and dietary fibre from tibetan spring barley in different ecological zones were analyzed.3-4 strains of the above materias were planted in Tibet Linzhi,the treatments of nitrogen, sowing date, dressing fertilizer rate,and so on were set, the sample contents ofβ-glucans and dietary fibre were determined, effect factors of different agronomy factors on contents ofβ-glucans and dietary fibre of barley. 8 strains that chemical content has significant differences on the above material were choice, planted 2 years in each production area of Tibetan barley, the ripened grain contents ofβ-glucans and dietary fibre were determined and analysed, the strains ,environment and interactive effects of the contents ofβ-glucans and dietary fibre were analysed by AMMI model,and distribution features of region were clear.The differences between strains and environment of the contents ofβ-glucans and dietary fibre of Tibetan barley were cleared by above research; some barley germplasms that contents ofβ-glucans and dietary fibre are high were selected. The effects of main climatic factors, agronomy factors and environmental condition on the contents ofβ-glucans and dietary fibre of Tibetan barley are studied, and main results are as follows:1. Effect of the climatic factors on the contents ofβ-glucans and dietary fibre①The effect of the main climatic factors on the contents ofβ-glucans on Tibetan barley grain is most cold monthly temperature, annual average sunshine class hours, greater than or equal 0℃annual accumulated temperature, altitude and so on. There were very significant correlations between the contents ofβ-glucans and most cold monthly temperature; there were significant negative correlations between the contents ofβ-glucans and annual average sunshine class hours. This shows that breeding the barley varieties that the contents ofβ-glucans are higher should mainly choose from the regions that greater than or equal 0℃annual accumulated temperature and annual average sunshine class hours are less, altitude and most cold monthly temperature are higher.②The effect of the main ecological character on the contents of dietary fibre on Tibetan barley grain is altitude, annual average sunshine class hours, greater than or equal 0℃annual accumulated temperature, annual average temperature, annual precipitation, most cold monthly temperature,and so on climatic factors. There were very significant correlations among the contents of dietary fibre, altitude, and annual average sunshine class hours, there were significant negative correlations among the contents of dietary fibre, annual precipitation and most cold monthly temperature. This shows that breeding the barley varieties that the contents of dietary fibre are higher should mainly choose from the regions that annual average sunshine class hours and altitude are higher, most cold monthly temperature and annual precipitation are less.2.Effect of agronomy factors on the contents ofβ-glucans and dietary fibre of Tibetan barley①The experiment result shows that the effect of sowing date onβ-glucan content was highest in this three agronomy factors, second was base fertilizer rate, and dressing fertilizer rate was relatively small. The suitable sowing date sof 4 varieties are about at April 10, Ximalaya 19 and Zangqing 320 may be properly early sowing, the suitable base fertilizer rate is 240kg/hm2. Ximalaya 19, Zangqing 320 and Shanqing 24 may be properly increased base fertilizer rate ,the suitable dressing fertilizer rate is from June 27 to July 2.②The experiment result shows that the effect of base fertilizer rate on dietary fibre content was highest in this three agronomy factors, second was dressing fertilizer rate, and sowing date was relatively small. The suitable sowing dates of 3 varieties are about at April 8, Ximalaya 19 may be properly early sowing, and the suitable base fertilizer rate is 220kg/hm2. Shanqing 24 may be properly increased base fertilizer rate, the suitable dressing fertilizer rate is from June 28 to July 2, and Ximalaya 19 may be properly early dressing fertilizer.3.Effects on genotype, environment and their interactive effects on content ofβ-glucans and dietary fibre from Tibetan barley①The variance ofβ-glucan content of Tibetan barley was attributable to the interactive effects between varieties and environment in this experiment, the second is varietal difference, and environmental effect was relatively small. Analysis of AMMI model showed that there were higherβ-glucan content in Shikatse and Lhong, and there were lessβ-glucan content in Changdu and Lhasa. The average content ofβ-glucans in variety 990852 from different ares was relatively low, PCA1 value was less, and the effect of environment was stable. The variety 990852 was relatively ideal variety.②The variance of dietary fibre content of Tibetan barley was attributable to environmental effect, the second is the interactive effects between varieties and environment, and varietal difference was relatively small. Analysis of AMMI model showed that there were higher dietary fibre content in Lhasa and Lhong, and there were less dietary fibre content in Changdu and Shikatse. The average content ofβ-glucans in Shanqing 23 and variety 990852 from different ares were relatively high, but PCA1 value was very little, therefore they were relatively ideal varieties .