| Insect GABA receptor is a target for pesticides and it is instructive to study the structure of insert GABA receptor proteins to develop efficient low-toxicity pesticides. In this paper, according to the latest research in material science, magnetic fluoresce nano-particles were prepared to separate housefly neuron cells and organism GABA receptor protein. The sharp of housefly neuron cells,the weight of housefly and mouse GABA receptor protein and their biological characteristics were researched..Magnetic fluoresce nano-particles were prepared by reverse microemulsion and ligand GABA was modified on the magnetic fluoresce nano-particles surface. Physical and chemical properties of magnetic fluoresce nano-particles were characterized by infrared spectroscopy, atomic force microscopy, transmission electron microscopy, vibrating sample magnetometer and BET surface area analyzer. Leukemia K562 cells as target cells,the biological safety of magnetic fluoresce nano-particles were detected, the result of which showed that magnetic fluoresce nano-particles were no toxicity on K562 cells.The ligand of magnetic fluoresce nano-particles were used to specific coupled with housefly GABA receptor which was on the surface of housefly neuron cells, housefly neuron cells which was labeled by magnetic fluoresce nano-particle complexes were separated under the magnetic field, the axon structure of neuron cells can be observed clearly under the fluorescence microscope, which was identified as neuron cells by collated the literature. GABA receptor proteins were gained after isolated neuron cells and purification and the molecular weight of which was determined by SDS-PAGE. Housefly GABA receptor protein, compared with mouse, the results of SDS-PAGE showed that the housefly and mouse GABA receptor both have three kinds of different subunits.The innovation of this paper is using magnetic fluoresce nano-particle complex to separate neuron cell and protein. |
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