High-through Detection Method Application In Maize Ssr Genetic Linkage Map Construction

Maize (Zea mays L.) is an important crop and feed in the world, which ranks first in all crops production. It is also most tractable genetic system among the cereals.Maize yield in China is second following the United States, which plays an important role in national economy. Thus, maize basical research and genetic improvement have great theoretical and practical significance.Construction of genetic map is the important elements of gene mapping and it is the basis of gene mapping, cloning and genome structure and function; Construction of maize genetic map and looking for molecular marker which is tightly linked to the target trait are the basis of molecular marker-assisted selection breeding. It is particularly important for QTL. New technology on molecular marker technique makes high-precision construction of genetic maps possible. Many crops highly saturated molecular linkage maps built help the further and deeper study of quantitative traits on the molecular level.DH population of maize (240 DH lines) were researched. It constructed a microsatellite or simple sequence repeats (simple sequence repeats, SSR) marker linkage map. Specific results are as follows:1.730 pairs of published PCR primers for amplifying microsatellite or simple sequence repeat (SSR) loci were used for the study.130 of them displayed polymorphism between the two parents. Chi-square test indicates that sixty-five markers (50% of all) skew from the expected gene segregation. Chromosomes 1,3, and 8 had outstanding performance.2. In order to improve the efficiency of Mapping Population of maize and to reduce the cost, high throughput measurement SSR electrophoresis technique was applied "Xian Yu 335" DH population. It process to multiple composite primers. This method was discussed in the maize targeted groups of SSR analysis.and provided a new way to improve the efficiency in maize molecular breeding.3.130 polymorphic loci are used to construct a genetic linkage map by the software Mapmaker/Exp3.0. Eventually a total of 119 sites which fit into the map were divided into 10 linkage groups. It covered a total length of 857.5cM with an average distance of 8.15cM between adjacent markers. The most one is Chromosomes 1 with 20 markers, and the least one is Chromosomes 6 with 8 markers. This mapping can be used to QTL research efficiently.