Research On The Relationship Between The Mrna Expression Of Serotonin 5-ht 4 Receptor Isoforms And Atriai Fibrillation In Atrial Myocardium

Objective To investigate the mRNA expression of four serotonin 5-HT4 receptor isoforms(5-HT4Ra,5-HT4Rb,5-HT4Rg,5-HT4Ri)in interatrial septum with atriaI fibriIIation (AF) and study its clinic significance.Methods Interatrial septums were obtained from 36 patients who underwent heart valve replacement. Among them, 17 patients were in sinus rhythm and 19 patients had AF. The mRNA expression of 5-HT4 receptor isoforms was measured by SYBR Green Real-time fluorescence quantitative PCR.Results 5-HT4Ra mRNA expression in the SR and AF Group was (19.89±9.68)×10-4 and (336.86±255.56 )×10-4 respectively. Compared with the SR Group, it was increased by 1593.61%(p<0.01)in the AF group; 5-HT4Rb mRNA expression in the SR and AF Group was (23.32±8.37)×10-4 and (240.32±242.49)×10-4 respectively. And it was increased by 930.53%(p<0.01)in the AF group; 5-HT4Rg mRNA expression in the SR and AF Group was (2.23±0.77)×10-4 and (0.72±0.45)×10-4 respectively. But it was decreased by 67.71%(p<0.01) in the AF group; 5-HT4Ri mRNA expression in the SR and AF Group was (34.95±8.08)×10-4 and (392.15±347.75)×10-4 respectively. And it was increased by 1022.03%(p<0.01)in the AF group.Conclusion These datas support that abnormal mRNA expression of 5-HT4 receptor isoforms in interatrial septum may be involved in the occurrence and maintenance of AF.