| BackgroundAcute cerebral ischemia is a very serious disease, which influences seriouslyhuman health and the quality of life. The free radical linkage reaction, caused bymiddle cerebral artery occlusion/reperfusion, is the main mechanism participating inbrain injury. To clear it and to inhibit its creation could obviously attenuate the braininjury and have marked protective effects. Brain-derived neurotrophic factor(BDNF),a member of neurotrophic factors family, was extracted from pig brain tissue byBarde in 1982. BDNF plays very important role to organism's growth, developmentand action maintenance of center nervous system. And it has significantneuroprotective effects in cerebral ischemia injury. Edaravone, also called 3-methyl-1-phenyl-2-pyrazolin- 5-one, is a new free radical scavenger developed by Japanese,used mainly in the therapy of acute cerebral ischemia. It appears as negative ion andcan give relief to brain injury through participate in generally several mechanisms ofacute cerebral ischemia. Erigeron Breviscapus is flavonoids abstracted fromcompositae plant. It has extensive roles in acute cerebral ischemia, myocardium I/Rinjury , blood viscidity and thrombocyte activity.Purpose1.To study the expression of free radicals and BDNF in cerebral cortex afterMCAO/R (middle cerebral artery occlusion/reperfusion ) injury in rats.2. To study the effect of Edaravone and Erigeron Breviscapus on the expression of free radicals and BDNF in cerebral cortex after MCAO/R injury, and to approachthe neuroprotective mechanism.MethodsA total of 96 healthy male rats were randomly divided into sham-operationgroup,control group, edaravone treated group and erigeron breviscapus treatedgroup(n=24 for each). Each group was further divided into 6h,12h,24h,48hsubgroups. The levels of SOD,MDA on each subgroup were examined. RT-PCRand western-blot were used to detect the expression of BDNF mRNA and proteinlevels, respectively.ResultsAfter MCAO/R injury, MDA content and SOD activity were statisticallysignificantly different between sham group and control group(P<0.05), and theexpression of BDNF mRNA and BDNF protein increased obviously compared withsham group. Edararone reduced MDA level and increased SOD activity signifiantlyversus control group(P<0.05). The expression of BDNF mRNA was statisticallysignificantly different between edaravone group and control group, so was theexpression of BDNF protein levels. The contents of MDA and SOD at 24h and 48hwere significantly different between erigeron breviscapus group and control group.But the expression of BDNF was not different between erigeron breviscapus groupand control group .ConclusionsThe expression of free radical and BDNF is increased after MCAO/R injury.Edaravone is a potent scavenger of free radicals. Edaravone increases the expression of BDNF after cerebral ischemia-reperfusion injury, which may play a new role in itsneuroprotection. Erigeron Breviscapus is a potent scavenger of free radicals. ErigeronBreviscapus show no effects on the expression of BDNF protein. |
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